Cautionary notes on the use of C-Terminal BAK1 fusion proteins for functional studies

Vardis Ntoukakis; Benjamin Schwessinger; Cécile Segonzac; Cyril Zipfel

doi:10.1105/tpc.111.090779

Cautionary notes on the use of C-Terminal BAK1 fusion proteins for functional studies

Vardis Ntoukakis, Benjamin Schwessinger, Cécile Segonzac, Cyril Zipfel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.

Original language	English
Pages (from-to)	3871-3878
Number of pages	8
Journal	Plant Cell
Volume	23
Issue number	11
DOIs	https://doi.org/10.1105/tpc.111.090779
Publication status	Published - Nov 2011
Externally published	Yes

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abstract = "Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.",

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AB - Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.

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Cautionary notes on the use of C-Terminal BAK1 fusion proteins for functional studies

Abstract

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