CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse

Florian Wiede; Phillip D. Fromm; Iain Comerford; Ervin Kara; Jennifer Bannan; Wolfgang Schuh; Charani Ranasinghe; David Tarlinton; Thomas Winkler; Shaun R. McColl; Heinrich Körner

doi:10.1038/icb.2013.14

CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse

Florian Wiede, Phillip D. Fromm, Iain Comerford, Ervin Kara, Jennifer Bannan, Wolfgang Schuh, Charani Ranasinghe, David Tarlinton, Thomas Winkler, Shaun R. McColl, Heinrich Körner^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The CC-chemokine receptor 6 (CCR6) is expressed constitutively at an intermediate level on naïve B cells and is upregulated after activation on pregerminal center (GC) B cells. We hypothesized that it could be involved in the events leading to GC reaction and high-affinity antibody production, and therefore investigated the potential role of CCR6 in B-cell differentiation in vivo. After antigenic challenge of CCR6^-/- mice with the T-cell-dependent antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH), GC B-cell development was found to be accelerated and the number of GC had increased significantly compared with control mice, but the antibodies produced by CCR6^-/- B cells were on average of lower affinity. We conclude from these data that the CCR6/CCL20 axis has an important role in regulating the kinetics and efficiency of the GC reaction.

Original language	English
Pages (from-to)	335-339
Number of pages	5
Journal	Immunology and Cell Biology
Volume	91
Issue number	5
DOIs	https://doi.org/10.1038/icb.2013.14
Publication status	Published - May 2013

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10.1038/icb.2013.14

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@article{cada1251316f41efa7071be9ee2d68c1,

title = "CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse",

abstract = "The CC-chemokine receptor 6 (CCR6) is expressed constitutively at an intermediate level on na{\"i}ve B cells and is upregulated after activation on pregerminal center (GC) B cells. We hypothesized that it could be involved in the events leading to GC reaction and high-affinity antibody production, and therefore investigated the potential role of CCR6 in B-cell differentiation in vivo. After antigenic challenge of CCR6-/- mice with the T-cell-dependent antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH), GC B-cell development was found to be accelerated and the number of GC had increased significantly compared with control mice, but the antibodies produced by CCR6-/- B cells were on average of lower affinity. We conclude from these data that the CCR6/CCL20 axis has an important role in regulating the kinetics and efficiency of the GC reaction.",

keywords = "B cells, chemokine receptor, germinal centers",

author = "Florian Wiede and Fromm, {Phillip D.} and Iain Comerford and Ervin Kara and Jennifer Bannan and Wolfgang Schuh and Charani Ranasinghe and David Tarlinton and Thomas Winkler and McColl, {Shaun R.} and Heinrich K{\"o}rner",

year = "2013",

month = may,

doi = "10.1038/icb.2013.14",

language = "English",

volume = "91",

pages = "335--339",

journal = "Immunology and Cell Biology",

issn = "0818-9641",

publisher = "John Wiley & Sons Inc.",

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TY - JOUR

T1 - CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse

AU - Wiede, Florian

AU - Fromm, Phillip D.

AU - Comerford, Iain

AU - Kara, Ervin

AU - Bannan, Jennifer

AU - Schuh, Wolfgang

AU - Ranasinghe, Charani

AU - Tarlinton, David

AU - Winkler, Thomas

AU - McColl, Shaun R.

AU - Körner, Heinrich

PY - 2013/5

Y1 - 2013/5

N2 - The CC-chemokine receptor 6 (CCR6) is expressed constitutively at an intermediate level on naïve B cells and is upregulated after activation on pregerminal center (GC) B cells. We hypothesized that it could be involved in the events leading to GC reaction and high-affinity antibody production, and therefore investigated the potential role of CCR6 in B-cell differentiation in vivo. After antigenic challenge of CCR6-/- mice with the T-cell-dependent antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH), GC B-cell development was found to be accelerated and the number of GC had increased significantly compared with control mice, but the antibodies produced by CCR6-/- B cells were on average of lower affinity. We conclude from these data that the CCR6/CCL20 axis has an important role in regulating the kinetics and efficiency of the GC reaction.

AB - The CC-chemokine receptor 6 (CCR6) is expressed constitutively at an intermediate level on naïve B cells and is upregulated after activation on pregerminal center (GC) B cells. We hypothesized that it could be involved in the events leading to GC reaction and high-affinity antibody production, and therefore investigated the potential role of CCR6 in B-cell differentiation in vivo. After antigenic challenge of CCR6-/- mice with the T-cell-dependent antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH), GC B-cell development was found to be accelerated and the number of GC had increased significantly compared with control mice, but the antibodies produced by CCR6-/- B cells were on average of lower affinity. We conclude from these data that the CCR6/CCL20 axis has an important role in regulating the kinetics and efficiency of the GC reaction.

KW - B cells

KW - chemokine receptor

KW - germinal centers

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U2 - 10.1038/icb.2013.14

DO - 10.1038/icb.2013.14

M3 - Article

SN - 0818-9641

VL - 91

SP - 335

EP - 339

JO - Immunology and Cell Biology

JF - Immunology and Cell Biology

IS - 5

ER -

CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse

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