CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse

Florian Wiede; Phillip D. Fromm; Iain Comerford; Ervin Kara; Jennifer Bannan; Wolfgang Schuh; Charani Ranasinghe; David Tarlinton; Thomas Winkler; Shaun R. McColl; Heinrich Körner

doi:10.1038/icb.2013.14

CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse

Florian Wiede
, Phillip D. Fromm
, Iain Comerford
, Ervin Kara
, Jennifer Bannan
, Wolfgang Schuh
, Charani Ranasinghe
, David Tarlinton
, Thomas Winkler
, Shaun R. McColl
, Heinrich Körner^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

The CC-chemokine receptor 6 (CCR6) is expressed constitutively at an intermediate level on naïve B cells and is upregulated after activation on pregerminal center (GC) B cells. We hypothesized that it could be involved in the events leading to GC reaction and high-affinity antibody production, and therefore investigated the potential role of CCR6 in B-cell differentiation in vivo. After antigenic challenge of CCR6^-/- mice with the T-cell-dependent antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH), GC B-cell development was found to be accelerated and the number of GC had increased significantly compared with control mice, but the antibodies produced by CCR6^-/- B cells were on average of lower affinity. We conclude from these data that the CCR6/CCL20 axis has an important role in regulating the kinetics and efficiency of the GC reaction.

Original language	English
Pages (from-to)	335-339
Number of pages	5
Journal	Immunology and Cell Biology
Volume	91
Issue number	5
DOIs	https://doi.org/10.1038/icb.2013.14
Publication status	Published - May 2013

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10.1038/icb.2013.14

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title = "CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse",

abstract = "The CC-chemokine receptor 6 (CCR6) is expressed constitutively at an intermediate level on na{\"i}ve B cells and is upregulated after activation on pregerminal center (GC) B cells. We hypothesized that it could be involved in the events leading to GC reaction and high-affinity antibody production, and therefore investigated the potential role of CCR6 in B-cell differentiation in vivo. After antigenic challenge of CCR6-/- mice with the T-cell-dependent antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH), GC B-cell development was found to be accelerated and the number of GC had increased significantly compared with control mice, but the antibodies produced by CCR6-/- B cells were on average of lower affinity. We conclude from these data that the CCR6/CCL20 axis has an important role in regulating the kinetics and efficiency of the GC reaction.",

keywords = "B cells, chemokine receptor, germinal centers",

author = "Florian Wiede and Fromm, \{Phillip D.\} and Iain Comerford and Ervin Kara and Jennifer Bannan and Wolfgang Schuh and Charani Ranasinghe and David Tarlinton and Thomas Winkler and McColl, \{Shaun R.\} and Heinrich K{\"o}rner",

year = "2013",

month = may,

doi = "10.1038/icb.2013.14",

language = "English",

volume = "91",

pages = "335--339",

journal = "Immunology and Cell Biology",

issn = "0818-9641",

publisher = "John Wiley \& Sons Inc.",

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TY - JOUR

T1 - CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse

AU - Wiede, Florian

AU - Fromm, Phillip D.

AU - Comerford, Iain

AU - Kara, Ervin

AU - Bannan, Jennifer

AU - Schuh, Wolfgang

AU - Ranasinghe, Charani

AU - Tarlinton, David

AU - Winkler, Thomas

AU - McColl, Shaun R.

AU - Körner, Heinrich

PY - 2013/5

Y1 - 2013/5

N2 - The CC-chemokine receptor 6 (CCR6) is expressed constitutively at an intermediate level on naïve B cells and is upregulated after activation on pregerminal center (GC) B cells. We hypothesized that it could be involved in the events leading to GC reaction and high-affinity antibody production, and therefore investigated the potential role of CCR6 in B-cell differentiation in vivo. After antigenic challenge of CCR6-/- mice with the T-cell-dependent antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH), GC B-cell development was found to be accelerated and the number of GC had increased significantly compared with control mice, but the antibodies produced by CCR6-/- B cells were on average of lower affinity. We conclude from these data that the CCR6/CCL20 axis has an important role in regulating the kinetics and efficiency of the GC reaction.

AB - The CC-chemokine receptor 6 (CCR6) is expressed constitutively at an intermediate level on naïve B cells and is upregulated after activation on pregerminal center (GC) B cells. We hypothesized that it could be involved in the events leading to GC reaction and high-affinity antibody production, and therefore investigated the potential role of CCR6 in B-cell differentiation in vivo. After antigenic challenge of CCR6-/- mice with the T-cell-dependent antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH), GC B-cell development was found to be accelerated and the number of GC had increased significantly compared with control mice, but the antibodies produced by CCR6-/- B cells were on average of lower affinity. We conclude from these data that the CCR6/CCL20 axis has an important role in regulating the kinetics and efficiency of the GC reaction.

KW - B cells

KW - chemokine receptor

KW - germinal centers

UR - https://www.scopus.com/pages/publications/84878548663

U2 - 10.1038/icb.2013.14

DO - 10.1038/icb.2013.14

M3 - Article

SN - 0818-9641

VL - 91

SP - 335

EP - 339

JO - Immunology and Cell Biology

JF - Immunology and Cell Biology

IS - 5

ER -

CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse

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