Abstract
Modern cell-free in vitro protein synthesis systems present powerful tools for the synthesis of isotope-labeled proteins in high yields. The production of selectively 15N-labeled proteins from 15N-labeled amino acids is particularly economic and yields are often sufficient to analyze the proteins very quickly by two-dimensional NMR spectra recorded of the crude reaction mixture without concentration or chromatographic purification of the protein. We review methodological aspects of cell-free in vitro protein synthesis based on an Escherichia coli cell extract, in particular with regard to the production of 15N-labeled proteins for analysis by NMR spectroscopy.
Original language | English |
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Pages (from-to) | 615-622 |
Number of pages | 8 |
Journal | IUBMB Life |
Volume | 57 |
Issue number | 9 |
DOIs | |
Publication status | Published - Sept 2005 |