Abstract
Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).
Original language | English |
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Pages (from-to) | 3356-3358 |
Number of pages | 3 |
Journal | Angewandte Chemie - International Edition |
Volume | 46 |
Issue number | 18 |
DOIs | |
Publication status | Published - 2007 |