Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

Peter S.C. Wu*, Kiyoshi Ozawa, Siew P. Lim, Subhash G. Vasudevan, Nicholas E. Dixon, Gottfried Otting

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    71 Citations (Scopus)

    Abstract

    Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).

    Original languageEnglish
    Pages (from-to)3356-3358
    Number of pages3
    JournalAngewandte Chemie - International Edition
    Volume46
    Issue number18
    DOIs
    Publication statusPublished - 2007

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