Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

Peter S.C. Wu; Kiyoshi Ozawa; Siew P. Lim; Subhash G. Vasudevan; Nicholas E. Dixon; Gottfried Otting

doi:10.1002/anie.200605237

Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

Peter S.C. Wu^*, Kiyoshi Ozawa, Siew P. Lim, Subhash G. Vasudevan, Nicholas E. Dixon, Gottfried Otting

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).

Original language	English
Pages (from-to)	3356-3358
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	46
Issue number	18
DOIs	https://doi.org/10.1002/anie.200605237
Publication status	Published - 2007

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10.1002/anie.200605237

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abstract = "Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).",

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AU - Wu, Peter S.C.

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AU - Lim, Siew P.

AU - Vasudevan, Subhash G.

AU - Dixon, Nicholas E.

AU - Otting, Gottfried

PY - 2007

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N2 - Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).

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KW - Cell-free protein synthesis

KW - High-throughput methods

KW - NMR spectroscopy

KW - Polymerase chain reaction

UR - https://www.scopus.com/pages/publications/34250826547

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DO - 10.1002/anie.200605237

M3 - Article

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JO - Angewandte Chemie - International Edition

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ER -

Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

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