Cell paintballing using optically targeted coacervate microdroplets

James P.K. Armstrong; Sam N. Olof; Monika D. Jakimowicz; Anthony P. Hollander; Stephen Mann; Sean A. Davis; Mervyn J. Miles; Avinash J. Patil; Adam W. Perriman

doi:10.1039/c5sc02266e

Cell paintballing using optically targeted coacervate microdroplets

James P.K. Armstrong^*, Sam N. Olof, Monika D. Jakimowicz, Anthony P. Hollander, Stephen Mann, Sean A. Davis, Mervyn J. Miles, Avinash J. Patil, Adam W. Perriman

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet-membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel "cell paintballing" technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.

Original language	English
Pages (from-to)	6106-6111
Number of pages	6
Journal	Chemical Science
Volume	6
Issue number	11
Early online date	20 Jul 2015
DOIs	https://doi.org/10.1039/c5sc02266e
Publication status	Published - 1 Nov 2015
Externally published	Yes

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10.1039/c5sc02266eLicence: CC BY

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title = "Cell paintballing using optically targeted coacervate microdroplets",

abstract = "We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet-membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel {"}cell paintballing{"} technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.",

author = "Armstrong, \{James P.K.\} and Olof, \{Sam N.\} and Jakimowicz, \{Monika D.\} and Hollander, \{Anthony P.\} and Stephen Mann and Davis, \{Sean A.\} and Miles, \{Mervyn J.\} and Patil, \{Avinash J.\} and Perriman, \{Adam W.\}",

note = "{\textcopyright} The Royal Society of Chemistry.",

year = "2015",

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doi = "10.1039/c5sc02266e",

language = "English",

volume = "6",

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TY - JOUR

T1 - Cell paintballing using optically targeted coacervate microdroplets

AU - Armstrong, James P.K.

AU - Olof, Sam N.

AU - Jakimowicz, Monika D.

AU - Hollander, Anthony P.

AU - Mann, Stephen

AU - Davis, Sean A.

AU - Miles, Mervyn J.

AU - Patil, Avinash J.

AU - Perriman, Adam W.

PY - 2015/11/1

Y1 - 2015/11/1

N2 - We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet-membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel "cell paintballing" technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.

AB - We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet-membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel "cell paintballing" technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.

UR - https://www.scopus.com/pages/publications/84944048837

U2 - 10.1039/c5sc02266e

DO - 10.1039/c5sc02266e

M3 - Article

AN - SCOPUS:84944048837

SN - 2041-6520

VL - 6

SP - 6106

EP - 6111

JO - Chemical Science

JF - Chemical Science

IS - 11

ER -

Cell paintballing using optically targeted coacervate microdroplets

Abstract

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