Characterization of Arabidopsis AtAMT2, a novel ammonium transporter in plants

Christian Sohlenkamp, Megan Shelden, Susan Howitt, Michael Udvardi*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    107 Citations (Scopus)

    Abstract

    We have cloned and characterized the first member of a novel family of ammonium transporters in plants: AtAMT2 from Arabidopsis thaliana. AtAMT2 is more closely related to bacterial ammonium transporters than to plant transporters of the AMT1 family. The protein was expressed and functionally characterized in yeast. AtAMT2 transported ammonium in an energy-dependent manner. In contrast to transporters of the AMT1 family, however, AtAMT2 did not transport the ammonium analogue, methylammonium. AtAMT2 was expressed more highly in shoots than roots and was subject to nitrogen regulation. Copyright (C) 2000 Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)273-278
    Number of pages6
    JournalFEBS Letters
    Volume467
    Issue number2-3
    DOIs
    Publication statusPublished - 11 Feb 2000

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