Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: Implications for stem cell biology and tissue repair

Ting Yi Wang; Kiara A.F. Bruggeman; Rebecca K. Sheean; Bradley J. Turner; David R. Nisbet; Clare L. Parish

doi:10.1074/jbc.M113.537381

Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: Implications for stem cell biology and tissue repair

Ting Yi Wang, Kiara A.F. Bruggeman, Rebecca K. Sheean, Bradley J. Turner, David R. Nisbet, Clare L. Parish^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Background: Tethering proteins onto bioengineered scaffolds enables longterm delivery, however protein stability, release kinetics, and functionality over time remains unknown. Results: Tethered proteins remain stable and functional for several months, capable of activating intracellular signaling pathways and influencing cell fate. Conclusion: Tethered proteins are stable and functional long-term. Significance: Such knowledge may have implications for promoting tissue repair.

Original language	English
Pages (from-to)	15044-15051
Number of pages	8
Journal	Journal of Biological Chemistry
Volume	289
Issue number	21
DOIs	https://doi.org/10.1074/jbc.M113.537381
Publication status	Published - 2014

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abstract = "Background: Tethering proteins onto bioengineered scaffolds enables longterm delivery, however protein stability, release kinetics, and functionality over time remains unknown. Results: Tethered proteins remain stable and functional for several months, capable of activating intracellular signaling pathways and influencing cell fate. Conclusion: Tethered proteins are stable and functional long-term. Significance: Such knowledge may have implications for promoting tissue repair.",

author = "Wang, {Ting Yi} and Bruggeman, {Kiara A.F.} and Sheean, {Rebecca K.} and Turner, {Bradley J.} and Nisbet, {David R.} and Parish, {Clare L.}",

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AU - Sheean, Rebecca K.

AU - Turner, Bradley J.

AU - Nisbet, David R.

AU - Parish, Clare L.

PY - 2014

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AB - Background: Tethering proteins onto bioengineered scaffolds enables longterm delivery, however protein stability, release kinetics, and functionality over time remains unknown. Results: Tethered proteins remain stable and functional for several months, capable of activating intracellular signaling pathways and influencing cell fate. Conclusion: Tethered proteins are stable and functional long-term. Significance: Such knowledge may have implications for promoting tissue repair.

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Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: Implications for stem cell biology and tissue repair

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