Chloroplast simple sequence repeat markers for evolutionary studies in the sexually deceptive orchid genus Chiloglottis

Daniel Ebert; Christine Hayes; Rod Peakall

doi:10.1111/j.1755-0998.2008.02321.x

Chloroplast simple sequence repeat markers for evolutionary studies in the sexually deceptive orchid genus Chiloglottis

Daniel Ebert, Christine Hayes, Rod Peakall^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The orchids in the genus Chiloglottis are pollinated exclusively by sexual deception. Extensive sequencing (> 19.5 kb) of noncoding chloroplast DNA revealed that simple sequence repeats (cpSSRs) were abundant, enabling a set of 41 cpSSR markers to be developed. All markers were polymorphic across the genus. Polymorphism reflected variation at both mononucleotide repeats and indels. For a subset of four taxa with 40 samples each, locus polymorphism varied from 46 to 81%, while the number of haplotypes ranged from seven to 21 per taxon. Extensive differentiation among the taxa was detected. These cpSSRs markers will enable novel insights into the evolution of this unique genus.

Original language	English
Pages (from-to)	784-789
Number of pages	6
Journal	Molecular Ecology Resources
Volume	9
Issue number	3
DOIs	https://doi.org/10.1111/j.1755-0998.2008.02321.x
Publication status	Published - May 2009

Access to Document

10.1111/j.1755-0998.2008.02321.x

Cite this

@article{61cf75c5cfa747a2945d8a11f7d605aa,

title = "Chloroplast simple sequence repeat markers for evolutionary studies in the sexually deceptive orchid genus Chiloglottis",

abstract = "The orchids in the genus Chiloglottis are pollinated exclusively by sexual deception. Extensive sequencing (> 19.5 kb) of noncoding chloroplast DNA revealed that simple sequence repeats (cpSSRs) were abundant, enabling a set of 41 cpSSR markers to be developed. All markers were polymorphic across the genus. Polymorphism reflected variation at both mononucleotide repeats and indels. For a subset of four taxa with 40 samples each, locus polymorphism varied from 46 to 81%, while the number of haplotypes ranged from seven to 21 per taxon. Extensive differentiation among the taxa was detected. These cpSSRs markers will enable novel insights into the evolution of this unique genus.",

keywords = "Chiloglottis, Chloroplast microsatellite, Chloroplast simple sequence repeat, CpSSR, Orchidaceae, Sexual deception",

author = "Daniel Ebert and Christine Hayes and Rod Peakall",

year = "2009",

month = may,

doi = "10.1111/j.1755-0998.2008.02321.x",

language = "English",

volume = "9",

pages = "784--789",

journal = "Molecular Ecology Resources",

issn = "1755-098X",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "3",

}

TY - JOUR

T1 - Chloroplast simple sequence repeat markers for evolutionary studies in the sexually deceptive orchid genus Chiloglottis

AU - Ebert, Daniel

AU - Hayes, Christine

AU - Peakall, Rod

PY - 2009/5

Y1 - 2009/5

N2 - The orchids in the genus Chiloglottis are pollinated exclusively by sexual deception. Extensive sequencing (> 19.5 kb) of noncoding chloroplast DNA revealed that simple sequence repeats (cpSSRs) were abundant, enabling a set of 41 cpSSR markers to be developed. All markers were polymorphic across the genus. Polymorphism reflected variation at both mononucleotide repeats and indels. For a subset of four taxa with 40 samples each, locus polymorphism varied from 46 to 81%, while the number of haplotypes ranged from seven to 21 per taxon. Extensive differentiation among the taxa was detected. These cpSSRs markers will enable novel insights into the evolution of this unique genus.

AB - The orchids in the genus Chiloglottis are pollinated exclusively by sexual deception. Extensive sequencing (> 19.5 kb) of noncoding chloroplast DNA revealed that simple sequence repeats (cpSSRs) were abundant, enabling a set of 41 cpSSR markers to be developed. All markers were polymorphic across the genus. Polymorphism reflected variation at both mononucleotide repeats and indels. For a subset of four taxa with 40 samples each, locus polymorphism varied from 46 to 81%, while the number of haplotypes ranged from seven to 21 per taxon. Extensive differentiation among the taxa was detected. These cpSSRs markers will enable novel insights into the evolution of this unique genus.

KW - Chiloglottis

KW - Chloroplast microsatellite

KW - Chloroplast simple sequence repeat

KW - CpSSR

KW - Orchidaceae

KW - Sexual deception

UR - http://www.scopus.com/inward/record.url?scp=65249147189&partnerID=8YFLogxK

U2 - 10.1111/j.1755-0998.2008.02321.x

DO - 10.1111/j.1755-0998.2008.02321.x

M3 - Article

SN - 1755-098X

VL - 9

SP - 784

EP - 789

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

IS - 3

ER -

Chloroplast simple sequence repeat markers for evolutionary studies in the sexually deceptive orchid genus Chiloglottis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this