Chromosome Analysis in Invertebrates and Vertebrates

David M. Rowell*, Shu Ly Lim, Frank Grutzner

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    8 Citations (Scopus)

    Abstract

    The revolution in molecular techniques over the last 30 years detracted from many traditional cytological techniques for examining basic biological problems. One of these casualties is the preparation of karyotypes and analysis of chromosomal structure, behaviour, and variation. Recent technology permitting the full sequencing of organisms has highlighted (but does not replace) the importance of understanding chromosomal constitution and karyotype structure, which underpin genome organisation. This chapter provides simple and straightforward protocols for the preparation of chromosome spreads from animals, and more advanced techniques for cell culture and chromosomal banding and hybridisation.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages13-35
    Number of pages23
    DOIs
    Publication statusPublished - 2011

    Publication series

    NameMethods in Molecular Biology
    Volume772
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

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