Clostridium carboxidivorans strain P7T recombinant formate dehydrogenase catalyzes reduction of CO2 to formate

Apostolos Alissandratos, Hye Kyung Kim, Hayden Matthews, James E. Hennessy, Amy Philbrook, Christopher J. Easton*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    73 Citations (Scopus)

    Abstract

    Recombinant formate dehydrogenase from the acetogen Clostridium carboxidivorans strain P7T, expressed in Escherichia coli, shows particular activity towards NADH-dependent carbon dioxide reduction to formate due to the relative binding affinities of the substrates and products. The enzyme retains activity over 2 days at 4°C under oxic conditions.

    Original languageEnglish
    Pages (from-to)741-744
    Number of pages4
    JournalApplied and Environmental Microbiology
    Volume79
    Issue number2
    DOIs
    Publication statusPublished - Jan 2013

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