Comparison of fluorotyrosines and methionine uptake in F98 rat gliomas

Karl Josef Langen; Michael Jarosch; Heinz Mühlensiepen; Kurt Hamacher; Stefan Bröer; Paul Jansen; Karl Zilles; Heinz H. Coenen

doi:10.1016/S0969-8051(03)00023-4

Comparison of fluorotyrosines and methionine uptake in F98 rat gliomas

Karl Josef Langen, Michael Jarosch, Heinz Mühlensiepen, Kurt Hamacher, Stefan Bröer, Paul Jansen, Karl Zilles, Heinz H. Coenen

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Abstract

The transport mechanisms of O-(2-[¹⁸F]fluoroethyl)-L-tyrosine (FET) and 2-[¹⁸F]fluoro-L-tyrosine (FTyr) were compared to those of [³H]-Methyl-L-methionine (MET) in F98 rat glioma cells in vitro and by tumor imaging by ex vivo dual tracer autoradiography in F98 rat gliomas. Both, FET and FTyr exhibited similar transport characteristics in F98 glioma cells compared to MET, i.e. mainly a sodium dependent transport similar to system B^0,+ and sodium independent transport via system L. Radioactivity of FET in the acid precipitable fraction was <1% after 120 min incubation time while FTyr and MET exhibited a 15-18% incorporation into proteins. Comparison of FET and FTyr with MET uptake in F98 rat gliomas demonstrated a significant correlation of tumor to brain ratios and a similar intratumoral tracer distribution pattern.

Original language	English
Pages (from-to)	501-508
Number of pages	8
Journal	Nuclear Medicine and Biology
Volume	30
Issue number	5
DOIs	https://doi.org/10.1016/S0969-8051(03)00023-4
Publication status	Published - May 2003

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title = "Comparison of fluorotyrosines and methionine uptake in F98 rat gliomas",

abstract = "The transport mechanisms of O-(2-[18F]fluoroethyl)-L-tyrosine (FET) and 2-[18F]fluoro-L-tyrosine (FTyr) were compared to those of [3H]-Methyl-L-methionine (MET) in F98 rat glioma cells in vitro and by tumor imaging by ex vivo dual tracer autoradiography in F98 rat gliomas. Both, FET and FTyr exhibited similar transport characteristics in F98 glioma cells compared to MET, i.e. mainly a sodium dependent transport similar to system B0,+ and sodium independent transport via system L. Radioactivity of FET in the acid precipitable fraction was <1% after 120 min incubation time while FTyr and MET exhibited a 15-18% incorporation into proteins. Comparison of FET and FTyr with MET uptake in F98 rat gliomas demonstrated a significant correlation of tumor to brain ratios and a similar intratumoral tracer distribution pattern.",

keywords = "2-[F]fluoro-L-tyrosine (FTyr), F98-glioma cells, O-(2-[F]fluoroethyl)-L-tyrosine (FET), PET, Radiolabelled amino acids, [H]-Methyl-L-methionine (MET)",

author = "Langen, {Karl Josef} and Michael Jarosch and Heinz M{\"u}hlensiepen and Kurt Hamacher and Stefan Br{\"o}er and Paul Jansen and Karl Zilles and Coenen, {Heinz H.}",

year = "2003",

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doi = "10.1016/S0969-8051(03)00023-4",

language = "English",

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AU - Langen, Karl Josef

AU - Jarosch, Michael

AU - Mühlensiepen, Heinz

AU - Hamacher, Kurt

AU - Bröer, Stefan

AU - Jansen, Paul

AU - Zilles, Karl

AU - Coenen, Heinz H.

PY - 2003/5

Y1 - 2003/5

N2 - The transport mechanisms of O-(2-[18F]fluoroethyl)-L-tyrosine (FET) and 2-[18F]fluoro-L-tyrosine (FTyr) were compared to those of [3H]-Methyl-L-methionine (MET) in F98 rat glioma cells in vitro and by tumor imaging by ex vivo dual tracer autoradiography in F98 rat gliomas. Both, FET and FTyr exhibited similar transport characteristics in F98 glioma cells compared to MET, i.e. mainly a sodium dependent transport similar to system B0,+ and sodium independent transport via system L. Radioactivity of FET in the acid precipitable fraction was <1% after 120 min incubation time while FTyr and MET exhibited a 15-18% incorporation into proteins. Comparison of FET and FTyr with MET uptake in F98 rat gliomas demonstrated a significant correlation of tumor to brain ratios and a similar intratumoral tracer distribution pattern.

AB - The transport mechanisms of O-(2-[18F]fluoroethyl)-L-tyrosine (FET) and 2-[18F]fluoro-L-tyrosine (FTyr) were compared to those of [3H]-Methyl-L-methionine (MET) in F98 rat glioma cells in vitro and by tumor imaging by ex vivo dual tracer autoradiography in F98 rat gliomas. Both, FET and FTyr exhibited similar transport characteristics in F98 glioma cells compared to MET, i.e. mainly a sodium dependent transport similar to system B0,+ and sodium independent transport via system L. Radioactivity of FET in the acid precipitable fraction was <1% after 120 min incubation time while FTyr and MET exhibited a 15-18% incorporation into proteins. Comparison of FET and FTyr with MET uptake in F98 rat gliomas demonstrated a significant correlation of tumor to brain ratios and a similar intratumoral tracer distribution pattern.

KW - 2-[F]fluoro-L-tyrosine (FTyr)

KW - F98-glioma cells

KW - O-(2-[F]fluoroethyl)-L-tyrosine (FET)

KW - PET

KW - Radiolabelled amino acids

KW - [H]-Methyl-L-methionine (MET)

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DO - 10.1016/S0969-8051(03)00023-4

M3 - Article

SN - 0969-8051

VL - 30

SP - 501

EP - 508

JO - Nuclear Medicine and Biology

JF - Nuclear Medicine and Biology

IS - 5

ER -

Comparison of fluorotyrosines and methionine uptake in F98 rat gliomas

Abstract

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