TY - JOUR
T1 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast
AU - Roguev, Assen
AU - Bandyopadhyay, Sourav
AU - Zofall, Martin
AU - Zhang, Ke
AU - Fischer, Tamas
AU - Collins, Sean R.
AU - Qu, Hongjing
AU - Shales, Michael
AU - Park, Han Oh
AU - Hayles, Jacqueline
AU - Hoe, Kwang Lae
AU - Kim, Dong Uk
AU - Ideker, Trey
AU - Grewal, Shiv I.
AU - Weissman, Jonathan S.
AU - Krogan, Nevan J.
PY - 2008/10/17
Y1 - 2008/10/17
N2 - An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway to several other biological processes. Comparison of the S. pombe E-MAP to an analogous genetic map from the budding yeast revealed that, whereas negative interactions were conserved between genes involved in similar biological processes, positive interactions and overall genetic profiles between pairs of genes coding for physically associated proteins were even more conserved. Hence, conservation occurs at the level of the functional module (protein complex), but the genetic cross talk between modules can differ substantially.
AB - An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway to several other biological processes. Comparison of the S. pombe E-MAP to an analogous genetic map from the budding yeast revealed that, whereas negative interactions were conserved between genes involved in similar biological processes, positive interactions and overall genetic profiles between pairs of genes coding for physically associated proteins were even more conserved. Hence, conservation occurs at the level of the functional module (protein complex), but the genetic cross talk between modules can differ substantially.
UR - http://www.scopus.com/inward/record.url?scp=54249107599&partnerID=8YFLogxK
U2 - 10.1126/science.1162609
DO - 10.1126/science.1162609
M3 - Article
SN - 0036-8075
VL - 322
SP - 405
EP - 410
JO - Science
JF - Science
IS - 5900
ER -