Construction of a multivalent vaccine strain of Shigella flexneri and evaluation of serotype-specfic immunity

Amy V. Jennison; Fleur Roberts; Naresh K. Verma

doi:10.1111/j.1574-695X.2006.00062.x

Construction of a multivalent vaccine strain of Shigella flexneri and evaluation of serotype-specfic immunity

Amy V. Jennison, Fleur Roberts, Naresh K. Verma^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Shigella flexneri causes more fatalities by shigellosis than any other Shigella species. There are 13 different serotypes of S. flexneri and their distribution varies between endemic geographical regions. The immune response against S. flexneri is serotype-specific, so current immunization strategies have required the administration of multiple vaccine strains to provide protection against multiple serotypes. In this study, we report the construction of a multivalent S. flexneri vaccine strain, SFL1425, expressing the O-antigen structure specific for serotypes 2a and 5a. This combination of type antigens has not previously been reported for S. flexneri. The multivalent vaccine strain, SFL1425 was able to induce a specific immune response against both serotypes 2a and 5a in a mouse pulmonary model.

Original language	English
Pages (from-to)	444-451
Number of pages	8
Journal	FEMS Immunology and Medical Microbiology
Volume	46
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-695X.2006.00062.x
Publication status	Published - Apr 2006

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title = "Construction of a multivalent vaccine strain of Shigella flexneri and evaluation of serotype-specfic immunity",

abstract = "Shigella flexneri causes more fatalities by shigellosis than any other Shigella species. There are 13 different serotypes of S. flexneri and their distribution varies between endemic geographical regions. The immune response against S. flexneri is serotype-specific, so current immunization strategies have required the administration of multiple vaccine strains to provide protection against multiple serotypes. In this study, we report the construction of a multivalent S. flexneri vaccine strain, SFL1425, expressing the O-antigen structure specific for serotypes 2a and 5a. This combination of type antigens has not previously been reported for S. flexneri. The multivalent vaccine strain, SFL1425 was able to induce a specific immune response against both serotypes 2a and 5a in a mouse pulmonary model.",

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AU - Roberts, Fleur

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AB - Shigella flexneri causes more fatalities by shigellosis than any other Shigella species. There are 13 different serotypes of S. flexneri and their distribution varies between endemic geographical regions. The immune response against S. flexneri is serotype-specific, so current immunization strategies have required the administration of multiple vaccine strains to provide protection against multiple serotypes. In this study, we report the construction of a multivalent S. flexneri vaccine strain, SFL1425, expressing the O-antigen structure specific for serotypes 2a and 5a. This combination of type antigens has not previously been reported for S. flexneri. The multivalent vaccine strain, SFL1425 was able to induce a specific immune response against both serotypes 2a and 5a in a mouse pulmonary model.

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Construction of a multivalent vaccine strain of Shigella flexneri and evaluation of serotype-specfic immunity

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