Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity

Vladimir Nekrasov; Jing Li; Martine Batoux; Milena Roux; Zhao Hui Chu; Severine Lacombe; Alejandra Rougon; Pascal Bittel; Marta Kiss-Papp; Delphine Chinchilla; H. Peter Van Esse; Lucia Jorda; Benjamin Schwessinger; Valerie Nicaise; Bart P.H.J. Thomma; Antonio Molina; Jonathan D.G. Jones; Cyril Zipfel

doi:10.1038/emboj.2009.262

Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity

Vladimir Nekrasov, Jing Li, Martine Batoux, Milena Roux, Zhao Hui Chu, Severine Lacombe, Alejandra Rougon, Pascal Bittel, Marta Kiss-Papp, Delphine Chinchilla, H. Peter Van Esse, Lucia Jorda, Benjamin Schwessinger, Valerie Nicaise, Bart P.H.J. Thomma, Antonio Molina, Jonathan D.G. Jones, Cyril Zipfel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

247 Citations (Scopus)

Abstract

In plant innate immunity, the surface-exposed leucine-rich repeat receptor kinases EFR and FLS2 mediate recognition of the bacterial pathogen-associated molecular patterns EF-Tu and flagellin, respectively. We identified the Arabidopsis stromal-derived factor-2 (SDF2) as being required for EFR function, and to a lesser extent FLS2 function. SDF2 resides in an endoplasmic reticulum (ER) protein complex with the Hsp40 ERdj3B and the Hsp70 BiP, which are components of the ER-quality control (ER-QC). Loss of SDF2 results in ER retention and degradation of EFR. The differential requirement for ER-QC components by EFR and FLS2 could be linked to N-glycosylation mediated by STT3a, a catalytic subunit of the oligosaccharyltransferase complex involved in co-translational N-glycosylation. Our results show that the plasma membrane EFR requires the ER complex SDF2-ERdj3B-BiP for its proper accumulation, and provide a demonstration of a physiological requirement for ER-QC in transmembrane receptor function in plants. They also provide an unexpected differential requirement for ER-QC and N-glycosylation components by two closely related receptors.

Original language	English
Pages (from-to)	3428-3438
Number of pages	11
Journal	EMBO Journal
Volume	28
Issue number	21
DOIs	https://doi.org/10.1038/emboj.2009.262
Publication status	Published - Nov 2009
Externally published	Yes

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10.1038/emboj.2009.262

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Nekrasov, V., Li, J., Batoux, M., Roux, M., Chu, Z. H., Lacombe, S., Rougon, A., Bittel, P., Kiss-Papp, M., Chinchilla, D., Van Esse, H. P., Jorda, L., Schwessinger, B., Nicaise, V., Thomma, B. P. H. J., Molina, A., Jones, J. D. G., & Zipfel, C. (2009). Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity. EMBO Journal, 28(21), 3428-3438. https://doi.org/10.1038/emboj.2009.262

@article{0bf67a57b03f42a7bf7fb6c291b8c5a0,

title = "Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity",

abstract = "In plant innate immunity, the surface-exposed leucine-rich repeat receptor kinases EFR and FLS2 mediate recognition of the bacterial pathogen-associated molecular patterns EF-Tu and flagellin, respectively. We identified the Arabidopsis stromal-derived factor-2 (SDF2) as being required for EFR function, and to a lesser extent FLS2 function. SDF2 resides in an endoplasmic reticulum (ER) protein complex with the Hsp40 ERdj3B and the Hsp70 BiP, which are components of the ER-quality control (ER-QC). Loss of SDF2 results in ER retention and degradation of EFR. The differential requirement for ER-QC components by EFR and FLS2 could be linked to N-glycosylation mediated by STT3a, a catalytic subunit of the oligosaccharyltransferase complex involved in co-translational N-glycosylation. Our results show that the plasma membrane EFR requires the ER complex SDF2-ERdj3B-BiP for its proper accumulation, and provide a demonstration of a physiological requirement for ER-QC in transmembrane receptor function in plants. They also provide an unexpected differential requirement for ER-QC and N-glycosylation components by two closely related receptors.",

keywords = "EFR, ER-quality control, Pathogen-associated molecular patterns, Pattern-recognition receptor, SDF2",

author = "Vladimir Nekrasov and Jing Li and Martine Batoux and Milena Roux and Chu, {Zhao Hui} and Severine Lacombe and Alejandra Rougon and Pascal Bittel and Marta Kiss-Papp and Delphine Chinchilla and {Van Esse}, {H. Peter} and Lucia Jorda and Benjamin Schwessinger and Valerie Nicaise and Thomma, {Bart P.H.J.} and Antonio Molina and Jones, {Jonathan D.G.} and Cyril Zipfel",

year = "2009",

month = nov,

doi = "10.1038/emboj.2009.262",

language = "English",

volume = "28",

pages = "3428--3438",

journal = "EMBO Journal",

issn = "0261-4189",

publisher = "Wiley-Blackwell",

number = "21",

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Nekrasov, V, Li, J, Batoux, M, Roux, M, Chu, ZH, Lacombe, S, Rougon, A, Bittel, P, Kiss-Papp, M, Chinchilla, D, Van Esse, HP, Jorda, L, Schwessinger, B, Nicaise, V, Thomma, BPHJ, Molina, A, Jones, JDG & Zipfel, C 2009, 'Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity', EMBO Journal, vol. 28, no. 21, pp. 3428-3438. https://doi.org/10.1038/emboj.2009.262

TY - JOUR

T1 - Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity

AU - Nekrasov, Vladimir

AU - Li, Jing

AU - Batoux, Martine

AU - Roux, Milena

AU - Chu, Zhao Hui

AU - Lacombe, Severine

AU - Rougon, Alejandra

AU - Bittel, Pascal

AU - Kiss-Papp, Marta

AU - Chinchilla, Delphine

AU - Van Esse, H. Peter

AU - Jorda, Lucia

AU - Schwessinger, Benjamin

AU - Nicaise, Valerie

AU - Thomma, Bart P.H.J.

AU - Molina, Antonio

AU - Jones, Jonathan D.G.

AU - Zipfel, Cyril

PY - 2009/11

Y1 - 2009/11

N2 - In plant innate immunity, the surface-exposed leucine-rich repeat receptor kinases EFR and FLS2 mediate recognition of the bacterial pathogen-associated molecular patterns EF-Tu and flagellin, respectively. We identified the Arabidopsis stromal-derived factor-2 (SDF2) as being required for EFR function, and to a lesser extent FLS2 function. SDF2 resides in an endoplasmic reticulum (ER) protein complex with the Hsp40 ERdj3B and the Hsp70 BiP, which are components of the ER-quality control (ER-QC). Loss of SDF2 results in ER retention and degradation of EFR. The differential requirement for ER-QC components by EFR and FLS2 could be linked to N-glycosylation mediated by STT3a, a catalytic subunit of the oligosaccharyltransferase complex involved in co-translational N-glycosylation. Our results show that the plasma membrane EFR requires the ER complex SDF2-ERdj3B-BiP for its proper accumulation, and provide a demonstration of a physiological requirement for ER-QC in transmembrane receptor function in plants. They also provide an unexpected differential requirement for ER-QC and N-glycosylation components by two closely related receptors.

AB - In plant innate immunity, the surface-exposed leucine-rich repeat receptor kinases EFR and FLS2 mediate recognition of the bacterial pathogen-associated molecular patterns EF-Tu and flagellin, respectively. We identified the Arabidopsis stromal-derived factor-2 (SDF2) as being required for EFR function, and to a lesser extent FLS2 function. SDF2 resides in an endoplasmic reticulum (ER) protein complex with the Hsp40 ERdj3B and the Hsp70 BiP, which are components of the ER-quality control (ER-QC). Loss of SDF2 results in ER retention and degradation of EFR. The differential requirement for ER-QC components by EFR and FLS2 could be linked to N-glycosylation mediated by STT3a, a catalytic subunit of the oligosaccharyltransferase complex involved in co-translational N-glycosylation. Our results show that the plasma membrane EFR requires the ER complex SDF2-ERdj3B-BiP for its proper accumulation, and provide a demonstration of a physiological requirement for ER-QC in transmembrane receptor function in plants. They also provide an unexpected differential requirement for ER-QC and N-glycosylation components by two closely related receptors.

KW - EFR

KW - ER-quality control

KW - Pathogen-associated molecular patterns

KW - Pattern-recognition receptor

KW - SDF2

UR - http://www.scopus.com/inward/record.url?scp=70350755586&partnerID=8YFLogxK

U2 - 10.1038/emboj.2009.262

DO - 10.1038/emboj.2009.262

M3 - Article

SN - 0261-4189

VL - 28

SP - 3428

EP - 3438

JO - EMBO Journal

JF - EMBO Journal

IS - 21

ER -

Control of the pattern-recognition receptor EFR by an ER protein complex in plant immunity

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