@inbook{082ca5bc27b9489a9319701f3bcd93b5,
title = "CRISPR/Cas9-Based Genome Editing of HSV",
abstract = "The CRISPR/Cas9 gene editing system is a robust and versatile technology that has revolutionized our capacity for genome engineering and is applicable in a wide range of organisms, including large dsDNA viruses. Here we provide an efficient methodology that can be used both for marker-based and for marker-free CRISPR/Cas9-mediated editing of the HSV-1 genome. In our method, Cas9, guide RNAs and a homology-directed repair template are provided to cells by cotransection of plasmids, followed by introduction of the HSV genome by infection. This method offers a great deal of flexibility, facilitating editing of the HSV genome that spans the range from individual nucleotide changes to large deletions and insertions.",
keywords = "CRISPR/Cas9, Genome editing, Herpes simplex virus, Homology-directed repair, Recombinant viruses",
author = "Thilaga Velusamy and Anjali Gowripalan and Tscharke, {David C.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-4939-9814-2_9",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "169--183",
booktitle = "Methods in Molecular Biology",
}