Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1

Li Wan; Simon J. Williams; Xiaoxiao Zhang; Daniel J. Ericsson; Markus Koeck; Peter N. Dodds; Jeffrey G. Ellis; Bostjan Kobe

doi:10.1107/S1744309113023142

Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1

Li Wan, Simon J. Williams, Xiaoxiao Zhang, Daniel J. Ericsson, Markus Koeck, Peter N. Dodds, Jeffrey G. Ellis, Bostjan Kobe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The plant hormones cytokinins play a central role in regulating cell division and developmental events. Cytokinin oxidase regulates the levels of these plant hormones by catalyzing their irreversible oxidation, which contributes to the regulation of various morpho-physiological processes controlled by cytokinins. In this study, the crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 are reported. Plate-like crystals of LuCKX1.1 were obtained using PEG 3350 as a precipitant and diffracted X-rays to 1.78 Å resolution. The protein crystals have the symmetry of space group C2 and are most likely to contain two molecules per asymmetric unit.

Original language	English
Pages (from-to)	1094-1096
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	69
Issue number	10
DOIs	https://doi.org/10.1107/S1744309113023142
Publication status	Published - Oct 2013
Externally published	Yes

Access to Document

10.1107/S1744309113023142

Cite this

@article{0976a7536cf141f5935f7d259bd50bb3,

title = "Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1",

abstract = "The plant hormones cytokinins play a central role in regulating cell division and developmental events. Cytokinin oxidase regulates the levels of these plant hormones by catalyzing their irreversible oxidation, which contributes to the regulation of various morpho-physiological processes controlled by cytokinins. In this study, the crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 are reported. Plate-like crystals of LuCKX1.1 were obtained using PEG 3350 as a precipitant and diffracted X-rays to 1.78 {\AA} resolution. The protein crystals have the symmetry of space group C2 and are most likely to contain two molecules per asymmetric unit.",

keywords = "cytokinin, cytokinin dehydrogenase, cytokinin oxidase, flavin adenine dinucleotide, plant hormones",

author = "Li Wan and Williams, {Simon J.} and Xiaoxiao Zhang and Ericsson, {Daniel J.} and Markus Koeck and Dodds, {Peter N.} and Ellis, {Jeffrey G.} and Bostjan Kobe",

year = "2013",

month = oct,

doi = "10.1107/S1744309113023142",

language = "English",

volume = "69",

pages = "1094--1096",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "John Wiley & Sons Ltd.",

number = "10",

}

TY - JOUR

T1 - Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1

AU - Wan, Li

AU - Williams, Simon J.

AU - Zhang, Xiaoxiao

AU - Ericsson, Daniel J.

AU - Koeck, Markus

AU - Dodds, Peter N.

AU - Ellis, Jeffrey G.

AU - Kobe, Bostjan

PY - 2013/10

Y1 - 2013/10

N2 - The plant hormones cytokinins play a central role in regulating cell division and developmental events. Cytokinin oxidase regulates the levels of these plant hormones by catalyzing their irreversible oxidation, which contributes to the regulation of various morpho-physiological processes controlled by cytokinins. In this study, the crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 are reported. Plate-like crystals of LuCKX1.1 were obtained using PEG 3350 as a precipitant and diffracted X-rays to 1.78 Å resolution. The protein crystals have the symmetry of space group C2 and are most likely to contain two molecules per asymmetric unit.

AB - The plant hormones cytokinins play a central role in regulating cell division and developmental events. Cytokinin oxidase regulates the levels of these plant hormones by catalyzing their irreversible oxidation, which contributes to the regulation of various morpho-physiological processes controlled by cytokinins. In this study, the crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 are reported. Plate-like crystals of LuCKX1.1 were obtained using PEG 3350 as a precipitant and diffracted X-rays to 1.78 Å resolution. The protein crystals have the symmetry of space group C2 and are most likely to contain two molecules per asymmetric unit.

KW - cytokinin

KW - cytokinin dehydrogenase

KW - cytokinin oxidase

KW - flavin adenine dinucleotide

KW - plant hormones

UR - http://www.scopus.com/inward/record.url?scp=84885455458&partnerID=8YFLogxK

U2 - 10.1107/S1744309113023142

DO - 10.1107/S1744309113023142

M3 - Article

SN - 1744-3091

VL - 69

SP - 1094

EP - 1096

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 10

ER -

Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1

Abstract

Access to Document

Other files and links

Fingerprint

Cite this