Detection of Helicobacteraceae in Intestinal Biopsies of Children with Crohn's Disease

Nadeem O. Kaakoush; Julie Holmes; Sophie Octavia; Si Ming Man; Li Zhang; Natalia Castaño-Rodríguez; Andrew S. Day; Steven T. Leach; Daniel A. Lemberg; Shoma Dutt; Michael Stormon; Edward V. O'Loughlin; Annabel Magoffin; Hazel Mitchell

doi:10.1111/j.1523-5378.2010.00792.x

Detection of Helicobacteraceae in Intestinal Biopsies of Children with Crohn's Disease

Nadeem O. Kaakoush, Julie Holmes, Sophie Octavia, Si Ming Man, Li Zhang, Natalia Castaño-Rodríguez, Andrew S. Day, Steven T. Leach, Daniel A. Lemberg, Shoma Dutt, Michael Stormon, Edward V. O'Loughlin, Annabel Magoffin, Hazel Mitchell^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Background: Given that members of Helicobacteraceae family colonize the intestinal mucus layer, it has been hypothesized that they may play a role in Crohn's disease. This study investigated the presence of Helicobacteraceae DNA in biopsies collected from children with Crohn's disease and controls.Materials and Methods: The presence of Helicobacteraceae DNA was investigated in intestinal biopsies collected from 179 children undergoing colonoscopy (Crohn's disease n = 77, controls n = 102) using a Helicobacteraceae-specific PCR.Results: Members of the Helicobacteraceae were detected in 32/77 children with Crohn's disease (41.5%) and 23/102 controls (22.5%). Statistical analysis showed the prevalence of Helicobacteraceae detected in patients to be significantly higher than that in controls (p =.0062). Analysis of non-pylori Helicobacteraceae showed that their prevalence was also significantly higher in patients than in controls (p =.04). Helicobacter pylori was detected in 14.0% of the biopsies across all groups. Given that all children tested were negative for gastric H. pylori, this was a surprising finding. Phylogenetic analysis of H. pylori sequences detected in the biopsies showed that the H. pylori strains identified in the patients did not group with gastric H. pylori included in the analysis, but rather with other H. pylori strains detected in the intestine, gall bladder, and liver.Conclusions: The higher prevalence of Helicobacteraceae DNA in Crohn's disease patients would suggest that members of this family may be involved in this disease. In addition, phylogenetic analysis of H. pylori strains showed that extragastric sequences clustered together, indicating that different H. pylori strains may adapt to colonize extragastric niches.

Original language	English
Pages (from-to)	549-557
Number of pages	9
Journal	Helicobacter
Volume	15
Issue number	6
DOIs	https://doi.org/10.1111/j.1523-5378.2010.00792.x
Publication status	Published - Dec 2010
Externally published	Yes

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Kaakoush, N. O., Holmes, J., Octavia, S., Man, S. M., Zhang, L., Castaño-Rodríguez, N., Day, A. S., Leach, S. T., Lemberg, D. A., Dutt, S., Stormon, M., O'Loughlin, E. V., Magoffin, A., & Mitchell, H. (2010). Detection of Helicobacteraceae in Intestinal Biopsies of Children with Crohn's Disease. Helicobacter, 15(6), 549-557. https://doi.org/10.1111/j.1523-5378.2010.00792.x

@article{0feeceb499ef4751bf2be2425fde241b,

title = "Detection of Helicobacteraceae in Intestinal Biopsies of Children with Crohn's Disease",

abstract = "Background: Given that members of Helicobacteraceae family colonize the intestinal mucus layer, it has been hypothesized that they may play a role in Crohn's disease. This study investigated the presence of Helicobacteraceae DNA in biopsies collected from children with Crohn's disease and controls.Materials and Methods: The presence of Helicobacteraceae DNA was investigated in intestinal biopsies collected from 179 children undergoing colonoscopy (Crohn's disease n = 77, controls n = 102) using a Helicobacteraceae-specific PCR.Results: Members of the Helicobacteraceae were detected in 32/77 children with Crohn's disease (41.5%) and 23/102 controls (22.5%). Statistical analysis showed the prevalence of Helicobacteraceae detected in patients to be significantly higher than that in controls (p =.0062). Analysis of non-pylori Helicobacteraceae showed that their prevalence was also significantly higher in patients than in controls (p =.04). Helicobacter pylori was detected in 14.0% of the biopsies across all groups. Given that all children tested were negative for gastric H. pylori, this was a surprising finding. Phylogenetic analysis of H. pylori sequences detected in the biopsies showed that the H. pylori strains identified in the patients did not group with gastric H. pylori included in the analysis, but rather with other H. pylori strains detected in the intestine, gall bladder, and liver.Conclusions: The higher prevalence of Helicobacteraceae DNA in Crohn's disease patients would suggest that members of this family may be involved in this disease. In addition, phylogenetic analysis of H. pylori strains showed that extragastric sequences clustered together, indicating that different H. pylori strains may adapt to colonize extragastric niches.",

keywords = "Crohn's disease, Detection, Helicobacteraceae, Phylogeny",

author = "Kaakoush, {Nadeem O.} and Julie Holmes and Sophie Octavia and Man, {Si Ming} and Li Zhang and Natalia Casta{\~n}o-Rodr{\'i}guez and Day, {Andrew S.} and Leach, {Steven T.} and Lemberg, {Daniel A.} and Shoma Dutt and Michael Stormon and O'Loughlin, {Edward V.} and Annabel Magoffin and Hazel Mitchell",

year = "2010",

month = dec,

doi = "10.1111/j.1523-5378.2010.00792.x",

language = "English",

volume = "15",

pages = "549--557",

journal = "Helicobacter",

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T1 - Detection of Helicobacteraceae in Intestinal Biopsies of Children with Crohn's Disease

AU - Kaakoush, Nadeem O.

AU - Holmes, Julie

AU - Octavia, Sophie

AU - Man, Si Ming

AU - Zhang, Li

AU - Castaño-Rodríguez, Natalia

AU - Day, Andrew S.

AU - Leach, Steven T.

AU - Lemberg, Daniel A.

AU - Dutt, Shoma

AU - Stormon, Michael

AU - O'Loughlin, Edward V.

AU - Magoffin, Annabel

AU - Mitchell, Hazel

PY - 2010/12

Y1 - 2010/12

N2 - Background: Given that members of Helicobacteraceae family colonize the intestinal mucus layer, it has been hypothesized that they may play a role in Crohn's disease. This study investigated the presence of Helicobacteraceae DNA in biopsies collected from children with Crohn's disease and controls.Materials and Methods: The presence of Helicobacteraceae DNA was investigated in intestinal biopsies collected from 179 children undergoing colonoscopy (Crohn's disease n = 77, controls n = 102) using a Helicobacteraceae-specific PCR.Results: Members of the Helicobacteraceae were detected in 32/77 children with Crohn's disease (41.5%) and 23/102 controls (22.5%). Statistical analysis showed the prevalence of Helicobacteraceae detected in patients to be significantly higher than that in controls (p =.0062). Analysis of non-pylori Helicobacteraceae showed that their prevalence was also significantly higher in patients than in controls (p =.04). Helicobacter pylori was detected in 14.0% of the biopsies across all groups. Given that all children tested were negative for gastric H. pylori, this was a surprising finding. Phylogenetic analysis of H. pylori sequences detected in the biopsies showed that the H. pylori strains identified in the patients did not group with gastric H. pylori included in the analysis, but rather with other H. pylori strains detected in the intestine, gall bladder, and liver.Conclusions: The higher prevalence of Helicobacteraceae DNA in Crohn's disease patients would suggest that members of this family may be involved in this disease. In addition, phylogenetic analysis of H. pylori strains showed that extragastric sequences clustered together, indicating that different H. pylori strains may adapt to colonize extragastric niches.

AB - Background: Given that members of Helicobacteraceae family colonize the intestinal mucus layer, it has been hypothesized that they may play a role in Crohn's disease. This study investigated the presence of Helicobacteraceae DNA in biopsies collected from children with Crohn's disease and controls.Materials and Methods: The presence of Helicobacteraceae DNA was investigated in intestinal biopsies collected from 179 children undergoing colonoscopy (Crohn's disease n = 77, controls n = 102) using a Helicobacteraceae-specific PCR.Results: Members of the Helicobacteraceae were detected in 32/77 children with Crohn's disease (41.5%) and 23/102 controls (22.5%). Statistical analysis showed the prevalence of Helicobacteraceae detected in patients to be significantly higher than that in controls (p =.0062). Analysis of non-pylori Helicobacteraceae showed that their prevalence was also significantly higher in patients than in controls (p =.04). Helicobacter pylori was detected in 14.0% of the biopsies across all groups. Given that all children tested were negative for gastric H. pylori, this was a surprising finding. Phylogenetic analysis of H. pylori sequences detected in the biopsies showed that the H. pylori strains identified in the patients did not group with gastric H. pylori included in the analysis, but rather with other H. pylori strains detected in the intestine, gall bladder, and liver.Conclusions: The higher prevalence of Helicobacteraceae DNA in Crohn's disease patients would suggest that members of this family may be involved in this disease. In addition, phylogenetic analysis of H. pylori strains showed that extragastric sequences clustered together, indicating that different H. pylori strains may adapt to colonize extragastric niches.

KW - Crohn's disease

KW - Detection

KW - Helicobacteraceae

KW - Phylogeny

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U2 - 10.1111/j.1523-5378.2010.00792.x

DO - 10.1111/j.1523-5378.2010.00792.x

M3 - Article

SN - 1083-4389

VL - 15

SP - 549

EP - 557

JO - Helicobacter

JF - Helicobacter

IS - 6

ER -

Detection of Helicobacteraceae in Intestinal Biopsies of Children with Crohn's Disease

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