Detection of mouse natural killer T follicular helper (NKTFH) cells by flow cytometry

Pheh Ping Chang; Carola G. Vinuesa

doi:10.1007/978-1-4939-2498-1_11

Detection of mouse natural killer T follicular helper (NKT_FH) cells by flow cytometry

Pheh Ping Chang^*, Carola G. Vinuesa

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Natural killer T follicular helper (NKT_FH) cell, a recently identified B-cell helper innate cell population, can be easily missed due to its low frequency and the fact that it only forms upon immunization or infection with glycolipid-containing antigens or microbes. Here, we describe our in-house optimized protocol to detect these mouse NKT_FH cells by multiparameter flow cytometer.

Original language	English
Title of host publication	T Follicular Helper Cells
Subtitle of host publication	Methods and Protocols
Publisher	Springer
Pages	135-141
Number of pages	7
ISBN (Electronic)	9781493924981
ISBN (Print)	9781493924974
DOIs	https://doi.org/10.1007/978-1-4939-2498-1_11
Publication status	Published - 2 Apr 2015

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abstract = "Natural killer T follicular helper (NKTFH) cell, a recently identified B-cell helper innate cell population, can be easily missed due to its low frequency and the fact that it only forms upon immunization or infection with glycolipid-containing antigens or microbes. Here, we describe our in-house optimized protocol to detect these mouse NKTFH cells by multiparameter flow cytometer.",

keywords = "Antibody staining, CD1d-α-GalCer tetramer, CXCR5, Flow cytometry (FACS), Natural killer T follicular helper (NKT) cells, PD-1",

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Detection of mouse natural killer T follicular helper (NKT_FH) cells by flow cytometry

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