Determination of amino acids in urine of patients with prostate cancer and benign prostate growth

Wiktor D. Sroka; Berin A. Boughton; Priyanka Reddy; Ute Roessner; Piotr Słupski; Piotr Jarzemski; Anita Dąbrowska; Michał J. Markuszewski; Michał P. Marszałł

doi:10.1097/CEJ.0000000000000248

Determination of amino acids in urine of patients with prostate cancer and benign prostate growth

Wiktor D. Sroka, Berin A. Boughton, Priyanka Reddy, Ute Roessner, Piotr Słupski, Piotr Jarzemski, Anita Dąbrowska, Michał J. Markuszewski, Michał P. Marszałł^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Prostate cancer is the leading type of cancer diagnosed in men. Serum prostate-specific antigen levels and digital rectal exam are far from perfect when it comes to differentiation of patients with prostate cancer and benign prostatic hyperplasia. In this study, we attempt to determine whether amino acids can be used as prostate cancer biomarkers. Concentrations of derivatized amino acids and amines were quantified by liquid chromatography tandem mass spectrometry. A total of 100 urine samples from the two groups including samples provided before and after prostate massage were examined quantitatively for amino acid and amine concentrations with 50 urine samples collected from cancer patients and 50 samples from patients diagnosed with benign prostatic hyperplasia. Arginine, homoserine, and proline were more abundant in urine samples of cancer patients compared with arginine, homoserine, and proline levels determined in urine collected from patients with benign growth. We also show that sarcosine is not a definitive indicator of prostate cancer when analyzed in urine samples collected either before or after prostate massage.

Original language	English
Pages (from-to)	131-134
Number of pages	4
Journal	European Journal of Cancer Prevention
Volume	26
Issue number	2
DOIs	https://doi.org/10.1097/CEJ.0000000000000248
Publication status	Published - 2017
Externally published	Yes

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title = "Determination of amino acids in urine of patients with prostate cancer and benign prostate growth",

abstract = "Prostate cancer is the leading type of cancer diagnosed in men. Serum prostate-specific antigen levels and digital rectal exam are far from perfect when it comes to differentiation of patients with prostate cancer and benign prostatic hyperplasia. In this study, we attempt to determine whether amino acids can be used as prostate cancer biomarkers. Concentrations of derivatized amino acids and amines were quantified by liquid chromatography tandem mass spectrometry. A total of 100 urine samples from the two groups including samples provided before and after prostate massage were examined quantitatively for amino acid and amine concentrations with 50 urine samples collected from cancer patients and 50 samples from patients diagnosed with benign prostatic hyperplasia. Arginine, homoserine, and proline were more abundant in urine samples of cancer patients compared with arginine, homoserine, and proline levels determined in urine collected from patients with benign growth. We also show that sarcosine is not a definitive indicator of prostate cancer when analyzed in urine samples collected either before or after prostate massage.",

keywords = "amino acids, benign prostatic hyperplasia, biomarker, cancer, prostate",

author = "Sroka, {Wiktor D.} and Boughton, {Berin A.} and Priyanka Reddy and Ute Roessner and Piotr S{\l}upski and Piotr Jarzemski and Anita D{\c a}browska and Markuszewski, {Micha{\l} J.} and Marsza{\l}{\l}, {Micha{\l} P.}",

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year = "2017",

doi = "10.1097/CEJ.0000000000000248",

language = "English",

volume = "26",

pages = "131--134",

journal = "European Journal of Cancer Prevention",

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T1 - Determination of amino acids in urine of patients with prostate cancer and benign prostate growth

AU - Sroka, Wiktor D.

AU - Boughton, Berin A.

AU - Reddy, Priyanka

AU - Roessner, Ute

AU - Słupski, Piotr

AU - Jarzemski, Piotr

AU - Dąbrowska, Anita

AU - Markuszewski, Michał J.

AU - Marszałł, Michał P.

PY - 2017

Y1 - 2017

N2 - Prostate cancer is the leading type of cancer diagnosed in men. Serum prostate-specific antigen levels and digital rectal exam are far from perfect when it comes to differentiation of patients with prostate cancer and benign prostatic hyperplasia. In this study, we attempt to determine whether amino acids can be used as prostate cancer biomarkers. Concentrations of derivatized amino acids and amines were quantified by liquid chromatography tandem mass spectrometry. A total of 100 urine samples from the two groups including samples provided before and after prostate massage were examined quantitatively for amino acid and amine concentrations with 50 urine samples collected from cancer patients and 50 samples from patients diagnosed with benign prostatic hyperplasia. Arginine, homoserine, and proline were more abundant in urine samples of cancer patients compared with arginine, homoserine, and proline levels determined in urine collected from patients with benign growth. We also show that sarcosine is not a definitive indicator of prostate cancer when analyzed in urine samples collected either before or after prostate massage.

AB - Prostate cancer is the leading type of cancer diagnosed in men. Serum prostate-specific antigen levels and digital rectal exam are far from perfect when it comes to differentiation of patients with prostate cancer and benign prostatic hyperplasia. In this study, we attempt to determine whether amino acids can be used as prostate cancer biomarkers. Concentrations of derivatized amino acids and amines were quantified by liquid chromatography tandem mass spectrometry. A total of 100 urine samples from the two groups including samples provided before and after prostate massage were examined quantitatively for amino acid and amine concentrations with 50 urine samples collected from cancer patients and 50 samples from patients diagnosed with benign prostatic hyperplasia. Arginine, homoserine, and proline were more abundant in urine samples of cancer patients compared with arginine, homoserine, and proline levels determined in urine collected from patients with benign growth. We also show that sarcosine is not a definitive indicator of prostate cancer when analyzed in urine samples collected either before or after prostate massage.

KW - amino acids

KW - benign prostatic hyperplasia

KW - biomarker

KW - cancer

KW - prostate

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DO - 10.1097/CEJ.0000000000000248

M3 - Article

SN - 0959-8278

VL - 26

SP - 131

EP - 134

JO - European Journal of Cancer Prevention

JF - European Journal of Cancer Prevention

IS - 2

ER -

Determination of amino acids in urine of patients with prostate cancer and benign prostate growth

Abstract

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