Determination of association constants of β-cyclodextrin and β-cyclodextrin-N-succinamate with drugs by high performance liquid chromatography

Alamdar Ashnagar, Christopher J. Easton, Nahid Gharib Naseri

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    Abstract

    Investigation of the Hummel and Dreyer method for the estimation of stability constants by high performance liquid chromatography (HPLC) is described. Comparison of the results obtained for binding of cimetidine (2a) and 2-(p-tolyl)ethylamine (2b) to β-cyclodextrin(1a) and β-cyclodextrin- N-succinamate (1b) by 1H NMR (300 MHz) spectroscopy and the HPLC method were studied. The results indicated that the stability constants by HPLC method are higher and more accurate, and are 431 and 470 dm3 mol -1 for (2a) and 138 and 111 dm3 mol-1 for (2b) in binding to (1a) and (1b), respectively.

    Original languageEnglish
    Pages (from-to)183-190
    Number of pages8
    JournalAsian Journal of Chemistry
    Volume17
    Issue number1
    Publication statusPublished - 2005

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