Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing

Olivier Clermont; Julia K. Christenson; Anne Sophie Daubié; David M. Gordon; Erick Denamur

doi:10.1016/j.mimet.2014.03.008

Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing

Olivier Clermont, Julia K. Christenson, Anne Sophie Daubié, David M. Gordon, Erick Denamur^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

Original language	English
Pages (from-to)	24-27
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	101
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2014.03.008
Publication status	Published - Jun 2014

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10.1016/j.mimet.2014.03.008

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title = "Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing",

abstract = "We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.",

keywords = "Allele-specific PCR, B2 phylogroup, B2 sub-group, Escherichia coli, Sequence type complex",

author = "Olivier Clermont and Christenson, \{Julia K.\} and Daubi{\'e}, \{Anne Sophie\} and Gordon, \{David M.\} and Erick Denamur",

year = "2014",

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AU - Clermont, Olivier

AU - Christenson, Julia K.

AU - Daubié, Anne Sophie

AU - Gordon, David M.

AU - Denamur, Erick

PY - 2014/6

Y1 - 2014/6

N2 - We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

AB - We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

KW - Allele-specific PCR

KW - B2 phylogroup

KW - B2 sub-group

KW - Escherichia coli

KW - Sequence type complex

UR - https://www.scopus.com/pages/publications/84898611621

U2 - 10.1016/j.mimet.2014.03.008

DO - 10.1016/j.mimet.2014.03.008

M3 - Article

SN - 0167-7012

VL - 101

SP - 24

EP - 27

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing

Abstract

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