TY - GEN
T1 - Differences in photoreceptor sensitivity to oxygen stress between long evans and sprague-dawley rats
AU - Chrysostomou, Vicki
AU - Stone, Jonathan
AU - Valter, Krisztina
PY - 2010
Y1 - 2010
N2 - Purpose: To examine the susceptibility of photoreceptors to hyperoxic stress in two rat strains, the pigmented Long Evans (LE) and the albino Sprague-Dawley (SD). Methods: Adult LE and SD rats were exposed to hyperoxia (75% oxygen) for 14 days. Retinas were assessed for electroretinogram (ERG) responses, cell death, and expression of a retinal stress factor. Results: In the LE strain, exposure to hyperoxia significantly reduced amplitudes of rod a-wave, rod b-wave and cone b-wave components of the ERG, and caused a 55-fold increase in photoreceptor cell death rates, and an upregulation of GFAP expression. In the SD strain, hyperoxic exposure had no measurable effect on the ERG response of rods or cones, and resulted in a modest (5-fold) increase in the rate of photoreceptor cell death. Conclusions: In LE and SD strains, hyperoxia induces cell death specific to photoreceptors. The effect is an order of magnitude more severe in the pigmented LE strain suggesting a strong genetic component to oxygen sensitivity, as reported previously between the albino Balb/C and pigmented C57BL/6 strains of mice.
AB - Purpose: To examine the susceptibility of photoreceptors to hyperoxic stress in two rat strains, the pigmented Long Evans (LE) and the albino Sprague-Dawley (SD). Methods: Adult LE and SD rats were exposed to hyperoxia (75% oxygen) for 14 days. Retinas were assessed for electroretinogram (ERG) responses, cell death, and expression of a retinal stress factor. Results: In the LE strain, exposure to hyperoxia significantly reduced amplitudes of rod a-wave, rod b-wave and cone b-wave components of the ERG, and caused a 55-fold increase in photoreceptor cell death rates, and an upregulation of GFAP expression. In the SD strain, hyperoxic exposure had no measurable effect on the ERG response of rods or cones, and resulted in a modest (5-fold) increase in the rate of photoreceptor cell death. Conclusions: In LE and SD strains, hyperoxia induces cell death specific to photoreceptors. The effect is an order of magnitude more severe in the pigmented LE strain suggesting a strong genetic component to oxygen sensitivity, as reported previously between the albino Balb/C and pigmented C57BL/6 strains of mice.
UR - http://www.scopus.com/inward/record.url?scp=79959613216&partnerID=8YFLogxK
U2 - 10.1007/978-1-4419-1399-9_54
DO - 10.1007/978-1-4419-1399-9_54
M3 - Conference contribution
SN - 9781441913982
T3 - Advances in Experimental Medicine and Biology
SP - 473
EP - 479
BT - Retinal Degenerative Diseases
A2 - Anderson, Robert
A2 - Mandal, Nawajes
A2 - Hollyfield, Joe
A2 - LaVail, Matthew
ER -