TY - JOUR
T1 - Differential Expression of Ly6C and T-bet Distinguish Effector and Memory Th1 CD4 + Cell Properties during Viral Infection
AU - Marshall, Heather D.
AU - Chandele, Anmol
AU - Jung, Yong Woo
AU - Meng, Hailong
AU - Poholek, Amanda C.
AU - Parish, Ian A.
AU - Rutishauser, Rachel
AU - Cui, Weiguo
AU - Kleinstein, Steven H.
AU - Craft, Joe
AU - Kaech, Susan M.
PY - 2011/10/28
Y1 - 2011/10/28
N2 - CD4 + T cells differentiate into multiple effector types, but it is unclear how they form memory T cells during infection in vivo. Profiling virus-specific CD4 + T cells revealed that effector cells with T helper 1 (Th1) or T follicular helper (Tfh) cell characteristics differentiated into memory cells, although expression of Tfh cell markers declined over time. In contrast to virus-specific effector CD8 + T cells, increased IL-7R expression was not a reliable marker of CD4 + memory precursor cells. However, decreased Ly6C and T-bet (Tbx21) expression distinguished a subset of Th1 cells that displayed greater longevity and proliferative responses to secondary infection. Moreover, the gene expression profile of Ly6C loT-bet int Th1 effector cells was virtually identical to mature memory CD4 + T cells, indicating early maturation of memory CD4 + T cell features in this subset during acute viral infection. This study provides a framework for memory CD4 + T cell development after acute viral infection.
AB - CD4 + T cells differentiate into multiple effector types, but it is unclear how they form memory T cells during infection in vivo. Profiling virus-specific CD4 + T cells revealed that effector cells with T helper 1 (Th1) or T follicular helper (Tfh) cell characteristics differentiated into memory cells, although expression of Tfh cell markers declined over time. In contrast to virus-specific effector CD8 + T cells, increased IL-7R expression was not a reliable marker of CD4 + memory precursor cells. However, decreased Ly6C and T-bet (Tbx21) expression distinguished a subset of Th1 cells that displayed greater longevity and proliferative responses to secondary infection. Moreover, the gene expression profile of Ly6C loT-bet int Th1 effector cells was virtually identical to mature memory CD4 + T cells, indicating early maturation of memory CD4 + T cell features in this subset during acute viral infection. This study provides a framework for memory CD4 + T cell development after acute viral infection.
UR - http://www.scopus.com/inward/record.url?scp=80755163540&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2011.08.016
DO - 10.1016/j.immuni.2011.08.016
M3 - Article
SN - 1074-7613
VL - 35
SP - 633
EP - 646
JO - Immunity
JF - Immunity
IS - 4
ER -