Differential Expression of Ly6C and T-bet Distinguish Effector and Memory Th1 CD4 + Cell Properties during Viral Infection

Heather D. Marshall, Anmol Chandele, Yong Woo Jung, Hailong Meng, Amanda C. Poholek, Ian A. Parish, Rachel Rutishauser, Weiguo Cui, Steven H. Kleinstein, Joe Craft, Susan M. Kaech*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

234 Citations (Scopus)

Abstract

CD4 + T cells differentiate into multiple effector types, but it is unclear how they form memory T cells during infection in vivo. Profiling virus-specific CD4 + T cells revealed that effector cells with T helper 1 (Th1) or T follicular helper (Tfh) cell characteristics differentiated into memory cells, although expression of Tfh cell markers declined over time. In contrast to virus-specific effector CD8 + T cells, increased IL-7R expression was not a reliable marker of CD4 + memory precursor cells. However, decreased Ly6C and T-bet (Tbx21) expression distinguished a subset of Th1 cells that displayed greater longevity and proliferative responses to secondary infection. Moreover, the gene expression profile of Ly6C loT-bet int Th1 effector cells was virtually identical to mature memory CD4 + T cells, indicating early maturation of memory CD4 + T cell features in this subset during acute viral infection. This study provides a framework for memory CD4 + T cell development after acute viral infection.

Original languageEnglish
Pages (from-to)633-646
Number of pages14
JournalImmunity
Volume35
Issue number4
DOIs
Publication statusPublished - 28 Oct 2011
Externally publishedYes

Fingerprint

Dive into the research topics of 'Differential Expression of Ly6C and T-bet Distinguish Effector and Memory Th1 CD4 + Cell Properties during Viral Infection'. Together they form a unique fingerprint.

Cite this