Direct detection of boldenone sulfate and glucuronide conjugates in horse urine by ion trap liquid chromatography-mass spectrometry

Fan Pu; Andrew R. McKinney; Allen M. Stenhouse; Craig J. Suann; Malcolm D. McLeod

doi:10.1016/j.jchromb.2004.09.052

Direct detection of boldenone sulfate and glucuronide conjugates in horse urine by ion trap liquid chromatography-mass spectrometry

Fan Pu, Andrew R. McKinney, Allen M. Stenhouse, Craig J. Suann, Malcolm D. McLeod

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

A study of the equine phase II metabolism of the anabolic agent boldenone is reported. Boldenone sulfate, boldenone glucuronide and their C17-epimers were synthesised as reference standards in our lab and a method was developed for their detection in a horse urine matrix. Solid phase extraction was used to purify the analytes, which were then detected by ion trap LC/MS. Negative and positive ionisation mode MS ² were used for the detection of sulfate and glucuronide conjugates, respectively. Boldenone sulfate and 17-epiboldenone glucuronide were detected as the major and minor phase II metabolites, respectively, in horse urine samples collected following the administration of boldenone undecylenate by intramuscular injection.

Original language	English
Pages (from-to)	241-246
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	813
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2004.09.052
Publication status	Published - 25 Dec 2004
Externally published	Yes

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10.1016/j.jchromb.2004.09.052

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title = "Direct detection of boldenone sulfate and glucuronide conjugates in horse urine by ion trap liquid chromatography-mass spectrometry",

abstract = "A study of the equine phase II metabolism of the anabolic agent boldenone is reported. Boldenone sulfate, boldenone glucuronide and their C17-epimers were synthesised as reference standards in our lab and a method was developed for their detection in a horse urine matrix. Solid phase extraction was used to purify the analytes, which were then detected by ion trap LC/MS. Negative and positive ionisation mode MS 2 were used for the detection of sulfate and glucuronide conjugates, respectively. Boldenone sulfate and 17-epiboldenone glucuronide were detected as the major and minor phase II metabolites, respectively, in horse urine samples collected following the administration of boldenone undecylenate by intramuscular injection.",

keywords = "Boldenone, Boldenone sulfate, Glucuronide",

author = "Fan Pu and McKinney, \{Andrew R.\} and Stenhouse, \{Allen M.\} and Suann, \{Craig J.\} and McLeod, \{Malcolm D.\}",

year = "2004",

month = dec,

day = "25",

doi = "10.1016/j.jchromb.2004.09.052",

language = "English",

volume = "813",

pages = "241--246",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier B.V.",

number = "1-2",

}

Direct detection of boldenone sulfate and glucuronide conjugates in horse urine by ion trap liquid chromatography-mass spectrometry. / Pu, Fan; McKinney, Andrew R.; Stenhouse, Allen M. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 813, No. 1-2, 25.12.2004, p. 241-246.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Direct detection of boldenone sulfate and glucuronide conjugates in horse urine by ion trap liquid chromatography-mass spectrometry

AU - Pu, Fan

AU - McKinney, Andrew R.

AU - Stenhouse, Allen M.

AU - Suann, Craig J.

AU - McLeod, Malcolm D.

PY - 2004/12/25

Y1 - 2004/12/25

N2 - A study of the equine phase II metabolism of the anabolic agent boldenone is reported. Boldenone sulfate, boldenone glucuronide and their C17-epimers were synthesised as reference standards in our lab and a method was developed for their detection in a horse urine matrix. Solid phase extraction was used to purify the analytes, which were then detected by ion trap LC/MS. Negative and positive ionisation mode MS 2 were used for the detection of sulfate and glucuronide conjugates, respectively. Boldenone sulfate and 17-epiboldenone glucuronide were detected as the major and minor phase II metabolites, respectively, in horse urine samples collected following the administration of boldenone undecylenate by intramuscular injection.

AB - A study of the equine phase II metabolism of the anabolic agent boldenone is reported. Boldenone sulfate, boldenone glucuronide and their C17-epimers were synthesised as reference standards in our lab and a method was developed for their detection in a horse urine matrix. Solid phase extraction was used to purify the analytes, which were then detected by ion trap LC/MS. Negative and positive ionisation mode MS 2 were used for the detection of sulfate and glucuronide conjugates, respectively. Boldenone sulfate and 17-epiboldenone glucuronide were detected as the major and minor phase II metabolites, respectively, in horse urine samples collected following the administration of boldenone undecylenate by intramuscular injection.

KW - Boldenone

KW - Boldenone sulfate

KW - Glucuronide

UR - https://www.scopus.com/pages/publications/8844256614

U2 - 10.1016/j.jchromb.2004.09.052

DO - 10.1016/j.jchromb.2004.09.052

M3 - Article

SN - 1570-0232

VL - 813

SP - 241

EP - 246

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1-2

ER -

Direct detection of boldenone sulfate and glucuronide conjugates in horse urine by ion trap liquid chromatography-mass spectrometry

Abstract

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