Direct regulation of shikimate, early phenylpropanoid, and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine

Luis Orduña; Miaomiao Li; David Navarro-Payá; Chen Zhang; Antonio Santiago; Pablo Romero; Živa Ramšak; Gabriele Magon; Janine Höll; Patrick Merz; Kristina Gruden; Alessandro Vannozzi; Dario Cantu; Jochen Bogs; Darren C.J. Wong; Shao shan Carol Huang; José Tomás Matus

doi:10.1111/tpj.15686

Direct regulation of shikimate, early phenylpropanoid, and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine

Luis Orduña
, Miaomiao Li
, David Navarro-Payá
, Chen Zhang
, Antonio Santiago
, Pablo Romero
, Živa Ramšak
, Gabriele Magon
, Janine Höll
, Patrick Merz
, Kristina Gruden
, Alessandro Vannozzi
, Dario Cantu
, Jochen Bogs
, Darren C.J. Wong
, Shao shan Carol Huang
, José Tomás Matus^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

Subgroup 2 R2R3 MYBs have been shown to upregulate stilbene synthase expression in grapevine; however, their broader roles in the regulation of secondary metabolism remained unclear. Combining whole genome gene co-expression networks, cistrome data for MYB13/14/15, and MYB15 overexpression we identified new regulatory targets across stilbenoid, early phenylpropanoid and shikimate pathways. We demonstrate the validity of this approach via the biochemical characterization of new ROMT targets and provide the networks and cistrome data to the community.

Original language	English
Pages (from-to)	529-547
Number of pages	19
Journal	Plant Journal
Volume	110
Issue number	2
DOIs	https://doi.org/10.1111/tpj.15686
Publication status	Published - Apr 2022

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10.1111/tpj.15686

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Orduña, L., Li, M., Navarro-Payá, D., Zhang, C., Santiago, A., Romero, P., Ramšak, Ž., Magon, G., Höll, J., Merz, P., Gruden, K., Vannozzi, A., Cantu, D., Bogs, J., Wong, D. C. J., Huang, S. S. C., & Matus, J. T. (2022). Direct regulation of shikimate, early phenylpropanoid, and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine. Plant Journal, 110(2), 529-547. https://doi.org/10.1111/tpj.15686

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title = "Direct regulation of shikimate, early phenylpropanoid, and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine",

abstract = "Subgroup 2 R2R3 MYBs have been shown to upregulate stilbene synthase expression in grapevine; however, their broader roles in the regulation of secondary metabolism remained unclear. Combining whole genome gene co-expression networks, cistrome data for MYB13/14/15, and MYB15 overexpression we identified new regulatory targets across stilbenoid, early phenylpropanoid and shikimate pathways. We demonstrate the validity of this approach via the biochemical characterization of new ROMT targets and provide the networks and cistrome data to the community.",

keywords = "DNA affinity purification sequencing, regulatory networks, secondary metabolism, transcription factors, transcriptional regulation",

author = "Luis Ordu{\~n}a and Miaomiao Li and David Navarro-Pay{\'a} and Chen Zhang and Antonio Santiago and Pablo Romero and {\v Z}iva Ram{\v s}ak and Gabriele Magon and Janine H{\"o}ll and Patrick Merz and Kristina Gruden and Alessandro Vannozzi and Dario Cantu and Jochen Bogs and Wong, \{Darren C.J.\} and Huang, \{Shao shan Carol\} and Matus, \{Jos{\'e} Tom{\'a}s\}",

year = "2022",

month = apr,

doi = "10.1111/tpj.15686",

language = "English",

volume = "110",

pages = "529--547",

journal = "Plant Journal",

issn = "0960-7412",

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Orduña, L, Li, M, Navarro-Payá, D, Zhang, C, Santiago, A, Romero, P, Ramšak, Ž, Magon, G, Höll, J, Merz, P, Gruden, K, Vannozzi, A, Cantu, D, Bogs, J, Wong, DCJ, Huang, SSC & Matus, JT 2022, 'Direct regulation of shikimate, early phenylpropanoid, and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine', Plant Journal, vol. 110, no. 2, pp. 529-547. https://doi.org/10.1111/tpj.15686

TY - JOUR

T1 - Direct regulation of shikimate, early phenylpropanoid, and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine

AU - Orduña, Luis

AU - Li, Miaomiao

AU - Navarro-Payá, David

AU - Zhang, Chen

AU - Santiago, Antonio

AU - Romero, Pablo

AU - Ramšak, Živa

AU - Magon, Gabriele

AU - Höll, Janine

AU - Merz, Patrick

AU - Gruden, Kristina

AU - Vannozzi, Alessandro

AU - Cantu, Dario

AU - Bogs, Jochen

AU - Wong, Darren C.J.

AU - Huang, Shao shan Carol

AU - Matus, José Tomás

PY - 2022/4

Y1 - 2022/4

N2 - Subgroup 2 R2R3 MYBs have been shown to upregulate stilbene synthase expression in grapevine; however, their broader roles in the regulation of secondary metabolism remained unclear. Combining whole genome gene co-expression networks, cistrome data for MYB13/14/15, and MYB15 overexpression we identified new regulatory targets across stilbenoid, early phenylpropanoid and shikimate pathways. We demonstrate the validity of this approach via the biochemical characterization of new ROMT targets and provide the networks and cistrome data to the community.

AB - Subgroup 2 R2R3 MYBs have been shown to upregulate stilbene synthase expression in grapevine; however, their broader roles in the regulation of secondary metabolism remained unclear. Combining whole genome gene co-expression networks, cistrome data for MYB13/14/15, and MYB15 overexpression we identified new regulatory targets across stilbenoid, early phenylpropanoid and shikimate pathways. We demonstrate the validity of this approach via the biochemical characterization of new ROMT targets and provide the networks and cistrome data to the community.

KW - DNA affinity purification sequencing

KW - regulatory networks

KW - secondary metabolism

KW - transcription factors

KW - transcriptional regulation

UR - https://www.scopus.com/pages/publications/85125544198

U2 - 10.1111/tpj.15686

DO - 10.1111/tpj.15686

M3 - Article

SN - 0960-7412

VL - 110

SP - 529

EP - 547

JO - Plant Journal

JF - Plant Journal

IS - 2

ER -

Direct regulation of shikimate, early phenylpropanoid, and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine

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