Disease stress-inducible genes of tobacco: Expression profile of elicitor-responsive genes isolated by subtractive hybridization

Daigo Takemoto*, Hirofumi Yoshioka, Noriyuki Doke, Kazuhito Kawakita

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    In order to investigate the change in mRNA profile during tobacco disease response, a subtractive hybridization procedure was used to generate a cDNA library for genes induced in tobacco (Nicotiana tabacum cv. Samsun NN) treated with oomycete elicitor. Database searches with the randomly isolated genes revealed that this cDNA library was enriched for reported disease stress-responsive genes such as pathogenesis-related proteins and cell wall protein genes. The expressions of eight newly isolated genes were induced by inoculation with the non-pathogenic bacteria, Pseudomonas syringae pv. glycinea. The NtEIGs (N. tabacum elicitor-inducible genes) showed similarity to genes for stellacyanin-like protein (NtEIG-A1), glutathione peroxidase (NtEIG-C08), extensin-like protein (NtEIG-C29), WRKY transcription factor (NtEIG-D48), glycine rich protein (NtEIG-E17), β-1, 3-glucanase-like protein (NtEIG-E76), photoassimilate-responsive protein-1 (NtEIG-E80) and wound-induced protein (NtEIG-D10). The expression patterns of NtEIGs in tobacco leaf in response to P. syringae pv. glycinea, salicylic acid (SA), methyl jasmonate (MeJA) and wound stress were analysed. The individual expression patterns of NtEIGs indicate that the transcriptional activation of NtEIGs is regulated by various signals and the products of NtEIGs are involved in different processes at different stages of the plant defence responses.

    Original languageEnglish
    Pages (from-to)545-553
    Number of pages9
    JournalPhysiologia Plantarum
    Volume118
    Issue number4
    DOIs
    Publication statusPublished - 1 Aug 2003

    Fingerprint

    Dive into the research topics of 'Disease stress-inducible genes of tobacco: Expression profile of elicitor-responsive genes isolated by subtractive hybridization'. Together they form a unique fingerprint.

    Cite this