DNA-drug systems: How circular dichroism data is complemented by other spectroscopic techniques

Alison Rodger; Harriet C. Latham; Paul Wormell; Andrew Parkinson; Matthew Ismail; Karen J. Sanders

DNA-drug systems: How circular dichroism data is complemented by other spectroscopic techniques

Alison Rodger^*, Harriet C. Latham, Paul Wormell, Andrew Parkinson, Matthew Ismail, Karen J. Sanders

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Circular dichroism (CD) measurements of DNA-drug complexes of two different systems are interpreted with the aid of other experimental and calculation data. The induced CD (ICD) of the polyamine mimic [Co(NH₃)]³⁺ has been used to determine the low drug:DNA ratio binding constant with calf thymus DNA of 1,00,000 M^-1 at 11 mM Na⁺. The site size is large. Linear dichroism (LD) shows how the DNA bends in accord with changes in DNA conformation observed in the CD. 9-hydroxyelliptine DNA binding in the intercalation limit and the higher concentration mode that gives rise to exciton CD signals have been probed using normal absorption, LD, both film and flow, and resonance light scattering. CNDO/S-CI calculations have also been investigated as a means of determining transition moments and intensities of individual transitions in a complex overlapping spectrum.

Original language	English
Pages (from-to)	395-408
Number of pages	14
Journal	Enantiomer
Volume	3
Issue number	4-5
Publication status	Published - 1998
Externally published	Yes

Cite this

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title = "DNA-drug systems: How circular dichroism data is complemented by other spectroscopic techniques",

abstract = "Circular dichroism (CD) measurements of DNA-drug complexes of two different systems are interpreted with the aid of other experimental and calculation data. The induced CD (ICD) of the polyamine mimic [Co(NH3)]3+ has been used to determine the low drug:DNA ratio binding constant with calf thymus DNA of 1,00,000 M-1 at 11 mM Na+. The site size is large. Linear dichroism (LD) shows how the DNA bends in accord with changes in DNA conformation observed in the CD. 9-hydroxyelliptine DNA binding in the intercalation limit and the higher concentration mode that gives rise to exciton CD signals have been probed using normal absorption, LD, both film and flow, and resonance light scattering. CNDO/S-CI calculations have also been investigated as a means of determining transition moments and intensities of individual transitions in a complex overlapping spectrum.",

keywords = "9-Hydroxyellipticine, Absorbance, Circular dichroism, Cobalt hexammine, DNA, Linear dichroism, Resonance light scattering, Spermine",

author = "Alison Rodger and Latham, {Harriet C.} and Paul Wormell and Andrew Parkinson and Matthew Ismail and Sanders, {Karen J.}",

year = "1998",

language = "English",

volume = "3",

pages = "395--408",

journal = "Enantiomer",

issn = "1024-2430",

publisher = "Taylor and Francis Ltd.",

number = "4-5",

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TY - JOUR

T1 - DNA-drug systems

T2 - How circular dichroism data is complemented by other spectroscopic techniques

AU - Rodger, Alison

AU - Latham, Harriet C.

AU - Wormell, Paul

AU - Parkinson, Andrew

AU - Ismail, Matthew

AU - Sanders, Karen J.

PY - 1998

Y1 - 1998

N2 - Circular dichroism (CD) measurements of DNA-drug complexes of two different systems are interpreted with the aid of other experimental and calculation data. The induced CD (ICD) of the polyamine mimic [Co(NH3)]3+ has been used to determine the low drug:DNA ratio binding constant with calf thymus DNA of 1,00,000 M-1 at 11 mM Na+. The site size is large. Linear dichroism (LD) shows how the DNA bends in accord with changes in DNA conformation observed in the CD. 9-hydroxyelliptine DNA binding in the intercalation limit and the higher concentration mode that gives rise to exciton CD signals have been probed using normal absorption, LD, both film and flow, and resonance light scattering. CNDO/S-CI calculations have also been investigated as a means of determining transition moments and intensities of individual transitions in a complex overlapping spectrum.

AB - Circular dichroism (CD) measurements of DNA-drug complexes of two different systems are interpreted with the aid of other experimental and calculation data. The induced CD (ICD) of the polyamine mimic [Co(NH3)]3+ has been used to determine the low drug:DNA ratio binding constant with calf thymus DNA of 1,00,000 M-1 at 11 mM Na+. The site size is large. Linear dichroism (LD) shows how the DNA bends in accord with changes in DNA conformation observed in the CD. 9-hydroxyelliptine DNA binding in the intercalation limit and the higher concentration mode that gives rise to exciton CD signals have been probed using normal absorption, LD, both film and flow, and resonance light scattering. CNDO/S-CI calculations have also been investigated as a means of determining transition moments and intensities of individual transitions in a complex overlapping spectrum.

KW - 9-Hydroxyellipticine

KW - Absorbance

KW - Circular dichroism

KW - Cobalt hexammine

KW - DNA

KW - Linear dichroism

KW - Resonance light scattering

KW - Spermine

UR - http://www.scopus.com/inward/record.url?scp=0032443325&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0032443325

SN - 1024-2430

VL - 3

SP - 395

EP - 408

JO - Enantiomer

JF - Enantiomer

IS - 4-5

ER -

DNA-drug systems: How circular dichroism data is complemented by other spectroscopic techniques

Abstract

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