TY - JOUR
T1 - Draft Genome Resource for Macrophomina phaseolina Associated with Charcoal Rot in Sorghum
AU - Purushotham, Neeraj
AU - Jones, Ashley
AU - Poudel, Barsha
AU - Nasim, Jamila
AU - Adorada, Dante
AU - Sparks, Adam
AU - Schwessinger, Benjamin
AU - Vaghefi, Niloofar
N1 - Publisher Copyright:
© 2020 The American Phytopathological Society
PY - 2020/5
Y1 - 2020/5
N2 - Macrophomina phaseolina is a soil-borne phytopathogenic fungus that causes charcoal rot in several plant species, including sorghum. We constructed a draft genome of M. phaseolina isolate BRIP 70780a from sorghum, using long-read native DNA from MinION sequencing, which was error-corrected using short-read Illumina MiSeq reads. The draft genome, consisting of 22 contigs with an N50 of 4,257,441 bp, 99.3% complete benchmarking universal single-copy orthologs, and 14,471 genes, is a valuable resource to aid future studies in population genomics and molecular diagnostic marker development for rapid detection of the pathogen.
AB - Macrophomina phaseolina is a soil-borne phytopathogenic fungus that causes charcoal rot in several plant species, including sorghum. We constructed a draft genome of M. phaseolina isolate BRIP 70780a from sorghum, using long-read native DNA from MinION sequencing, which was error-corrected using short-read Illumina MiSeq reads. The draft genome, consisting of 22 contigs with an N50 of 4,257,441 bp, 99.3% complete benchmarking universal single-copy orthologs, and 14,471 genes, is a valuable resource to aid future studies in population genomics and molecular diagnostic marker development for rapid detection of the pathogen.
KW - Charcoal rot
KW - Macrophomina phaseolina
KW - MinION
KW - Oxford Nanopore
KW - Sorghum
UR - http://www.scopus.com/inward/record.url?scp=85084130419&partnerID=8YFLogxK
U2 - 10.1094/MPMI-12-19-0356-A
DO - 10.1094/MPMI-12-19-0356-A
M3 - Article
SN - 0894-0282
VL - 33
SP - 724
EP - 726
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 5
ER -