Dynamic complex optical fields for optical manipulation, 3D microscopy and photostimulation of neurotransmitters

Vincent Ricardo Daria, Christian Stricker, John Bekkers, Steve Redman, Hans Bachor

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    We demonstrate a multi-functional system capable of multiple-site two-photon excitation of photo-sensitive compounds as well as transfer of optical mechanical properties on an array of mesoscopic particles. We use holographic projection of a single Ti:Sapphire laser operating in femtosecond pulse mode to show that the projected three-dimensional light patterns have sufficient spatiotemporal photon density for multi-site two-photon excitation of biological fluorescent markers and caged neurotransmitters. Using the same laser operating in continuous-wave mode we can use the same light patterns for non-invasive transfer of both linear and orbital angular momentum on a variety of mesoscopic particles. The system also incorporates high-speed scanning using acousto-optic modulators to rapidly render 3D images of neuron samples via two-photon microscopy.

    Original languageEnglish
    Title of host publicationOptical Trapping and Optical Micromanipulation VII
    DOIs
    Publication statusPublished - 2010
    EventOptical Trapping and Optical Micromanipulation VII - San Diego, CA, United States
    Duration: 1 Aug 20105 Aug 2010

    Publication series

    NameProceedings of SPIE - The International Society for Optical Engineering
    Volume7762
    ISSN (Print)0277-786X

    Conference

    ConferenceOptical Trapping and Optical Micromanipulation VII
    Country/TerritoryUnited States
    CitySan Diego, CA
    Period1/08/105/08/10

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