Effect of Photobiomodulation on Proliferative Changes in the Retina: Evidence from an In Vitro Model of PVR

Oscar Watson; Riemke Aggio-Bruce; Rohan Essex; Krisztina Valter

doi:10.1007/978-3-031-76550-6_32

Effect of Photobiomodulation on Proliferative Changes in the Retina: Evidence from an In Vitro Model of PVR

Oscar Watson
, Riemke Aggio-Bruce
, Rohan Essex
, Krisztina Valter

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We aimed to validate an in vitro model of proliferative vitreoretinopathy (PVR) and investigate the effect of 670 nm light photobiomodulation (PBM) on PVR pathophysiology.Immortalised retinal pigment epithelial cells (ARPE-19) were seeded at 3.0 × 103 cells/well. Half were stimulated with TGF-β1 to induce EMT, then all cells were allocated to either PBM, one treatment of 670 nm light every 3 hours for 12 hours or control. EMT morphology was characterised by area, perimeter, perimeter-area ratio, maximum Feret diameter, minimum Feret diameter, Feret ratio, compactness, eccentricity, and radius. The same parameters were analysed to determine the effect of PBM on EMT morphology.TGF-β induced morphological changes in ARPE-19 consistent with EMT, namely increased eccentricity, compactness, maximum Feret diameter, Feret ratio and perimeter-area ratio. Treatment with 670 nm PBM mitigates changes in perimeter and maximum Feret diameter induced by EMT.The TGF-β1 stimulation of RPE cells produces a morphologically valid in vitro model of EMT. PBM is effective at mitigating some EMT-related morphological changes.

Original language	English
Pages (from-to)	195-200
Number of pages	6
Journal	Advances in Experimental Medicine and Biology
Volume	1468
DOIs	https://doi.org/10.1007/978-3-031-76550-6_32
Publication status	Published - 2025

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@article{f7e2a540910a40ee8f897e070cf0763f,

title = "Effect of Photobiomodulation on Proliferative Changes in the Retina: Evidence from an In Vitro Model of PVR",

abstract = "We aimed to validate an in vitro model of proliferative vitreoretinopathy (PVR) and investigate the effect of 670 nm light photobiomodulation (PBM) on PVR pathophysiology.Immortalised retinal pigment epithelial cells (ARPE-19) were seeded at 3.0 × 103 cells/well. Half were stimulated with TGF-β1 to induce EMT, then all cells were allocated to either PBM, one treatment of 670 nm light every 3 hours for 12 hours or control. EMT morphology was characterised by area, perimeter, perimeter-area ratio, maximum Feret diameter, minimum Feret diameter, Feret ratio, compactness, eccentricity, and radius. The same parameters were analysed to determine the effect of PBM on EMT morphology.TGF-β induced morphological changes in ARPE-19 consistent with EMT, namely increased eccentricity, compactness, maximum Feret diameter, Feret ratio and perimeter-area ratio. Treatment with 670 nm PBM mitigates changes in perimeter and maximum Feret diameter induced by EMT.The TGF-β1 stimulation of RPE cells produces a morphologically valid in vitro model of EMT. PBM is effective at mitigating some EMT-related morphological changes.",

keywords = "ARPE-19, CellProfiler, Epithelial-mesenchymal transition, Photobiomodulation (PBM), Proliferative vitreoretinopathy, Rhegmatogenous retinal detachment, TGF-β",

author = "Oscar Watson and Riemke Aggio-Bruce and Rohan Essex and Krisztina Valter",

note = "{\textcopyright} 2025. The Author(s)",

year = "2025",

doi = "10.1007/978-3-031-76550-6\_32",

language = "English",

volume = "1468",

pages = "195--200",

journal = "Advances in Experimental Medicine and Biology",

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T1 - Effect of Photobiomodulation on Proliferative Changes in the Retina

T2 - Evidence from an In Vitro Model of PVR

AU - Watson, Oscar

AU - Aggio-Bruce, Riemke

AU - Essex, Rohan

AU - Valter, Krisztina

PY - 2025

Y1 - 2025

N2 - We aimed to validate an in vitro model of proliferative vitreoretinopathy (PVR) and investigate the effect of 670 nm light photobiomodulation (PBM) on PVR pathophysiology.Immortalised retinal pigment epithelial cells (ARPE-19) were seeded at 3.0 × 103 cells/well. Half were stimulated with TGF-β1 to induce EMT, then all cells were allocated to either PBM, one treatment of 670 nm light every 3 hours for 12 hours or control. EMT morphology was characterised by area, perimeter, perimeter-area ratio, maximum Feret diameter, minimum Feret diameter, Feret ratio, compactness, eccentricity, and radius. The same parameters were analysed to determine the effect of PBM on EMT morphology.TGF-β induced morphological changes in ARPE-19 consistent with EMT, namely increased eccentricity, compactness, maximum Feret diameter, Feret ratio and perimeter-area ratio. Treatment with 670 nm PBM mitigates changes in perimeter and maximum Feret diameter induced by EMT.The TGF-β1 stimulation of RPE cells produces a morphologically valid in vitro model of EMT. PBM is effective at mitigating some EMT-related morphological changes.

AB - We aimed to validate an in vitro model of proliferative vitreoretinopathy (PVR) and investigate the effect of 670 nm light photobiomodulation (PBM) on PVR pathophysiology.Immortalised retinal pigment epithelial cells (ARPE-19) were seeded at 3.0 × 103 cells/well. Half were stimulated with TGF-β1 to induce EMT, then all cells were allocated to either PBM, one treatment of 670 nm light every 3 hours for 12 hours or control. EMT morphology was characterised by area, perimeter, perimeter-area ratio, maximum Feret diameter, minimum Feret diameter, Feret ratio, compactness, eccentricity, and radius. The same parameters were analysed to determine the effect of PBM on EMT morphology.TGF-β induced morphological changes in ARPE-19 consistent with EMT, namely increased eccentricity, compactness, maximum Feret diameter, Feret ratio and perimeter-area ratio. Treatment with 670 nm PBM mitigates changes in perimeter and maximum Feret diameter induced by EMT.The TGF-β1 stimulation of RPE cells produces a morphologically valid in vitro model of EMT. PBM is effective at mitigating some EMT-related morphological changes.

KW - ARPE-19

KW - CellProfiler

KW - Epithelial-mesenchymal transition

KW - Photobiomodulation (PBM)

KW - Proliferative vitreoretinopathy

KW - Rhegmatogenous retinal detachment

KW - TGF-β

UR - https://www.scopus.com/pages/publications/85218438959

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DO - 10.1007/978-3-031-76550-6_32

M3 - Article

C2 - 39930195

AN - SCOPUS:85218438959

SN - 0065-2598

VL - 1468

SP - 195

EP - 200

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

ER -

Effect of Photobiomodulation on Proliferative Changes in the Retina: Evidence from an In Vitro Model of PVR

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