Effective priming of herpes simplex virusspecific CD8+ T cells in vivo does not require infected dendritic cells

Paul G. Whitney, Christina Makhlouf, Beth MacLeod, Joel Z. Ma, Elise Gressier, Marie Greyer, Katharina Hochheiser, Annabell Bachem, Ali Zaid, David Voehringer, William R. Heath, Mayura V. Wagle, Ian Parish, Tiffany A. Russell, Stewart A. Smith, David C. Tscharke, Thomas Gebhardt*, Sammy Bedoui

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    Resolution of virus infections depends on the priming of virus-specific CD8+ T cells by dendritic cells (DC). While this process requires major histocompatibility complex (MHC) class I-restricted antigen presentation by DC, the relative contribution to CD8+ T cell priming by infected DC is less clear. We have addressed this question in the context of a peripheral infection with herpes simplex virus 1 (HSV). Assessing the endogenous, polyclonal HSV-specific CD8+ T cell response, we found that effective in vivo T cell priming depended on the presence of DC subsets specialized in cross-presentation, while Langerhans cells and plasmacytoid DC were dispensable. Utilizing a novel mouse model that allows for the in vivo elimination of infected DC, we also demonstrated in vivo that this requirement for cross-presenting DC was not related to their infection but instead reflected their capacity to crosspresent HSV-derived antigen. Taking the results together, this study shows that infected DC are not required for effective CD8+ T cell priming during a peripheral virus infection.

    Original languageEnglish
    Article numbere01508-17
    JournalJournal of Virology
    Volume92
    Issue number3
    DOIs
    Publication statusPublished - 1 Feb 2018

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