Effects of pentobarbital on GABA-activated currents in acutely-isolated rat dentate gyrus granule neurons

M. S.F. Lim; C. E.L. Lindquist; B. Birnir

doi:10.1016/j.neulet.2003.09.025

Effects of pentobarbital on GABA-activated currents in acutely-isolated rat dentate gyrus granule neurons

M. S.F. Lim, C. E.L. Lindquist, B. Birnir^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Granule neurons from the rat dentate gyrus were acutely isolated and whole-cell currents recorded. Maximal enhancement of 7 μM γ-aminobutyric acid (GABA; EC₃₀) evoked currents was obtained with 100 μM pentobarbital where the peak-current was 2.1±0.2 of control. One hundred μM pentobarbital alone evoked no current response whereas 1 mM pentobarbital elicited a current response that was 0.4±0.2 of the 100 μM GABA-activated peak current. In 100 μM pentobarbital, the GABA EC₅₀ value shifted from 14 to 3 μM but the peak-saturating-current value was not altered. An off-current was recorded on removal of 100 μM and higher pentobarbital concentrations. Ten mM pentobarbital abolished the peak-current response to 7 and 100 μM GABA. The results show that in the granule neurons the drug potency differs for the different effects of pentobarbital at GABA_A receptors with the modulatory and inhibitory effects requiring lower concentrations than the direct activation of the receptors.

Original language	English
Pages (from-to)	139-142
Number of pages	4
Journal	Neuroscience Letters
Volume	353
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2003.09.025
Publication status	Published - 19 Dec 2003

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title = "Effects of pentobarbital on GABA-activated currents in acutely-isolated rat dentate gyrus granule neurons",

abstract = "Granule neurons from the rat dentate gyrus were acutely isolated and whole-cell currents recorded. Maximal enhancement of 7 μM γ-aminobutyric acid (GABA; EC30) evoked currents was obtained with 100 μM pentobarbital where the peak-current was 2.1±0.2 of control. One hundred μM pentobarbital alone evoked no current response whereas 1 mM pentobarbital elicited a current response that was 0.4±0.2 of the 100 μM GABA-activated peak current. In 100 μM pentobarbital, the GABA EC50 value shifted from 14 to 3 μM but the peak-saturating-current value was not altered. An off-current was recorded on removal of 100 μM and higher pentobarbital concentrations. Ten mM pentobarbital abolished the peak-current response to 7 and 100 μM GABA. The results show that in the granule neurons the drug potency differs for the different effects of pentobarbital at GABAA receptors with the modulatory and inhibitory effects requiring lower concentrations than the direct activation of the receptors.",

keywords = "Barbiturates, Hippocampus, Inhibition, Ion channel, Receptor, γ-aminobutyric acid",

author = "Lim, {M. S.F.} and Lindquist, {C. E.L.} and B. Birnir",

year = "2003",

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AU - Lim, M. S.F.

AU - Lindquist, C. E.L.

AU - Birnir, B.

PY - 2003/12/19

Y1 - 2003/12/19

N2 - Granule neurons from the rat dentate gyrus were acutely isolated and whole-cell currents recorded. Maximal enhancement of 7 μM γ-aminobutyric acid (GABA; EC30) evoked currents was obtained with 100 μM pentobarbital where the peak-current was 2.1±0.2 of control. One hundred μM pentobarbital alone evoked no current response whereas 1 mM pentobarbital elicited a current response that was 0.4±0.2 of the 100 μM GABA-activated peak current. In 100 μM pentobarbital, the GABA EC50 value shifted from 14 to 3 μM but the peak-saturating-current value was not altered. An off-current was recorded on removal of 100 μM and higher pentobarbital concentrations. Ten mM pentobarbital abolished the peak-current response to 7 and 100 μM GABA. The results show that in the granule neurons the drug potency differs for the different effects of pentobarbital at GABAA receptors with the modulatory and inhibitory effects requiring lower concentrations than the direct activation of the receptors.

AB - Granule neurons from the rat dentate gyrus were acutely isolated and whole-cell currents recorded. Maximal enhancement of 7 μM γ-aminobutyric acid (GABA; EC30) evoked currents was obtained with 100 μM pentobarbital where the peak-current was 2.1±0.2 of control. One hundred μM pentobarbital alone evoked no current response whereas 1 mM pentobarbital elicited a current response that was 0.4±0.2 of the 100 μM GABA-activated peak current. In 100 μM pentobarbital, the GABA EC50 value shifted from 14 to 3 μM but the peak-saturating-current value was not altered. An off-current was recorded on removal of 100 μM and higher pentobarbital concentrations. Ten mM pentobarbital abolished the peak-current response to 7 and 100 μM GABA. The results show that in the granule neurons the drug potency differs for the different effects of pentobarbital at GABAA receptors with the modulatory and inhibitory effects requiring lower concentrations than the direct activation of the receptors.

KW - Barbiturates

KW - Hippocampus

KW - Inhibition

KW - Ion channel

KW - Receptor

KW - γ-aminobutyric acid

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SP - 139

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JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2

ER -

Effects of pentobarbital on GABA-activated currents in acutely-isolated rat dentate gyrus granule neurons

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