Effects of surfactants on the formation of microdroplets in the flow focusing microfluidic device

Nan Wu; Y. Zhu; P. W. Leech; B. A. Sexton; S. Brown; C. Easton

doi:10.1117/12.769326

Effects of surfactants on the formation of microdroplets in the flow focusing microfluidic device

Nan Wu^*, Y. Zhu, P. W. Leech, B. A. Sexton, S. Brown, C. Easton

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

9 Citations (Scopus)

Abstract

Droplet-based in vitro compartmentalization (IVC) platform is a powerful tool in protein analysis. Reliable formation of microdroplets is important for the development of the microfluidic chip. In this study, we will examine the effect of surfactants on the formation of microdroplets in the flow focusing microfluidic device which is needed for enzyme evolution. Surfactants of Span 80 and Tween 20 are used in the continuous and dispersed phases, respectively. The droplet formation was affected distinguishably with the presence of Span 80 and Tween 20. The size of droplets decreased as the concentration of Span 80 increased. And the decrease was more pronounced with the combination of Span 80 and Tween 20.

Original language	English
Title of host publication	BioMEMS and Nanotechnology III
DOIs	https://doi.org/10.1117/12.769326
Publication status	Published - 2008
Event	BioMEMS and Nanotechnology III - Canberra, ACT, Australia Duration: 5 Dec 2007 → 7 Dec 2007

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6799
ISSN (Print)	1605-7422

Conference

Conference	BioMEMS and Nanotechnology III
Country/Territory	Australia
City	Canberra, ACT
Period	5/12/07 → 7/12/07

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10.1117/12.769326

Cite this

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title = "Effects of surfactants on the formation of microdroplets in the flow focusing microfluidic device",

abstract = "Droplet-based in vitro compartmentalization (IVC) platform is a powerful tool in protein analysis. Reliable formation of microdroplets is important for the development of the microfluidic chip. In this study, we will examine the effect of surfactants on the formation of microdroplets in the flow focusing microfluidic device which is needed for enzyme evolution. Surfactants of Span 80 and Tween 20 are used in the continuous and dispersed phases, respectively. The droplet formation was affected distinguishably with the presence of Span 80 and Tween 20. The size of droplets decreased as the concentration of Span 80 increased. And the decrease was more pronounced with the combination of Span 80 and Tween 20.",

keywords = "Enzyme evolution, Microdroplets, Microfluidic, Span80, Tween20",

author = "Nan Wu and Y. Zhu and Leech, {P. W.} and Sexton, {B. A.} and S. Brown and C. Easton",

year = "2008",

doi = "10.1117/12.769326",

language = "English",

isbn = "9780819469700",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "BioMEMS and Nanotechnology III",

note = "BioMEMS and Nanotechnology III ; Conference date: 05-12-2007 Through 07-12-2007",

}

Wu, N, Zhu, Y, Leech, PW, Sexton, BA, Brown, S & Easton, C 2008, Effects of surfactants on the formation of microdroplets in the flow focusing microfluidic device. in BioMEMS and Nanotechnology III., 67990C, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6799, BioMEMS and Nanotechnology III, Canberra, ACT, Australia, 5/12/07. https://doi.org/10.1117/12.769326

TY - GEN

T1 - Effects of surfactants on the formation of microdroplets in the flow focusing microfluidic device

AU - Wu, Nan

AU - Zhu, Y.

AU - Leech, P. W.

AU - Sexton, B. A.

AU - Brown, S.

AU - Easton, C.

PY - 2008

Y1 - 2008

N2 - Droplet-based in vitro compartmentalization (IVC) platform is a powerful tool in protein analysis. Reliable formation of microdroplets is important for the development of the microfluidic chip. In this study, we will examine the effect of surfactants on the formation of microdroplets in the flow focusing microfluidic device which is needed for enzyme evolution. Surfactants of Span 80 and Tween 20 are used in the continuous and dispersed phases, respectively. The droplet formation was affected distinguishably with the presence of Span 80 and Tween 20. The size of droplets decreased as the concentration of Span 80 increased. And the decrease was more pronounced with the combination of Span 80 and Tween 20.

AB - Droplet-based in vitro compartmentalization (IVC) platform is a powerful tool in protein analysis. Reliable formation of microdroplets is important for the development of the microfluidic chip. In this study, we will examine the effect of surfactants on the formation of microdroplets in the flow focusing microfluidic device which is needed for enzyme evolution. Surfactants of Span 80 and Tween 20 are used in the continuous and dispersed phases, respectively. The droplet formation was affected distinguishably with the presence of Span 80 and Tween 20. The size of droplets decreased as the concentration of Span 80 increased. And the decrease was more pronounced with the combination of Span 80 and Tween 20.

KW - Enzyme evolution

KW - Microdroplets

KW - Microfluidic

KW - Span80

KW - Tween20

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U2 - 10.1117/12.769326

DO - 10.1117/12.769326

M3 - Conference contribution

SN - 9780819469700

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - BioMEMS and Nanotechnology III

T2 - BioMEMS and Nanotechnology III

Y2 - 5 December 2007 through 7 December 2007

ER -

Effects of surfactants on the formation of microdroplets in the flow focusing microfluidic device

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