Efficient SNP-sensitive alignment and database-assisted SNP calling for low coverage samples

Ruibang Luo*, Chi Man Liu, Thomas Wong, Chang Yu, Tak Wah Lam, Siu Ming Yiu, Ruiqiang Li, Hing Fung Ting

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    We have designed and implemented an efficient tool for short read alignment that is sensitive to a given set of SNP. In particular, it returns alignments that permit mismatches at these SNPs. We then make use of it to develop a method for detecting SNPs, which allows user to provide annotated SNPs classified in previous studies and use them to guide the execution. By focusing on alignments covering these SNPs, our method greatly accelerates the detection of SNPs at prescribed loci. The annotated SNPs also help us distinguish sequencing errors from authentic SNP alleles easily. We have compared our method with existing methods on several applications. We found that our method have higher accuracy, especially for samples with low coverage. It is faster and can be about two orders of magnitude faster for some applications.

    Original languageEnglish
    Title of host publication2012 ACM Conference on Bioinformatics, Computational Biology and Biomedicine, BCB 2012
    Pages163-169
    Number of pages7
    DOIs
    Publication statusPublished - 2012
    Event2012 ACM Conference on Bioinformatics, Computational Biology and Biomedicine, BCB 2012 - Orlando, FL, United States
    Duration: 7 Oct 201210 Oct 2012

    Publication series

    Name2012 ACM Conference on Bioinformatics, Computational Biology and Biomedicine, BCB 2012

    Conference

    Conference2012 ACM Conference on Bioinformatics, Computational Biology and Biomedicine, BCB 2012
    Country/TerritoryUnited States
    CityOrlando, FL
    Period7/10/1210/10/12

    Cite this