Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

Feng Li; Narayana M. Upadhyaya; Jana Sperschneider; Oadi Matny; Hoa Nguyen-Phuc; Rohit Mago; Castle Raley; Marisa E. Miller; Kevin A.T. Silverstein; Eva Henningsen; Cory D. Hirsch; Botma Visser; Zacharias A. Pretorius; Brian J. Steffenson; Benjamin Schwessinger; Peter N. Dodds; Melania Figueroa

doi:10.1038/s41467-019-12927-7

Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

Feng Li, Narayana M. Upadhyaya, Jana Sperschneider, Oadi Matny, Hoa Nguyen-Phuc, Rohit Mago, Castle Raley, Marisa E. Miller, Kevin A.T. Silverstein, Eva Henningsen, Cory D. Hirsch, Botma Visser, Zacharias A. Pretorius, Brian J. Steffenson, Benjamin Schwessinger, Peter N. Dodds^*, Melania Figueroa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

128 Citations (Scopus)

Abstract

Parasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However, non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), such as Ug99, are a major threat to global food security. Here, we provide genomics-based evidence supporting that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons. Fully haplotype-resolved genome assembly and DNA proximity analysis reveal that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt, with no recombination or chromosome reassortment. These findings indicate that nuclear exchange between dikaryotes can generate genetic diversity and facilitate the emergence of new lineages in asexual fungal populations.

Original language	English
Article number	5068
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-12927-7
Publication status	Published - 1 Dec 2019

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Li, F., Upadhyaya, N. M., Sperschneider, J., Matny, O., Nguyen-Phuc, H., Mago, R., Raley, C., Miller, M. E., Silverstein, K. A. T., Henningsen, E., Hirsch, C. D., Visser, B., Pretorius, Z. A., Steffenson, B. J., Schwessinger, B., Dodds, P. N., & Figueroa, M. (2019). Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation. Nature Communications, 10(1), Article 5068. https://doi.org/10.1038/s41467-019-12927-7

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title = "Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation",

abstract = "Parasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However, non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), such as Ug99, are a major threat to global food security. Here, we provide genomics-based evidence supporting that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons. Fully haplotype-resolved genome assembly and DNA proximity analysis reveal that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt, with no recombination or chromosome reassortment. These findings indicate that nuclear exchange between dikaryotes can generate genetic diversity and facilitate the emergence of new lineages in asexual fungal populations.",

author = "Feng Li and Upadhyaya, \{Narayana M.\} and Jana Sperschneider and Oadi Matny and Hoa Nguyen-Phuc and Rohit Mago and Castle Raley and Miller, \{Marisa E.\} and Silverstein, \{Kevin A.T.\} and Eva Henningsen and Hirsch, \{Cory D.\} and Botma Visser and Pretorius, \{Zacharias A.\} and Steffenson, \{Brian J.\} and Benjamin Schwessinger and Dodds, \{Peter N.\} and Melania Figueroa",

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doi = "10.1038/s41467-019-12927-7",

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Li, F, Upadhyaya, NM, Sperschneider, J, Matny, O, Nguyen-Phuc, H, Mago, R, Raley, C, Miller, ME, Silverstein, KAT, Henningsen, E, Hirsch, CD, Visser, B, Pretorius, ZA, Steffenson, BJ, Schwessinger, B, Dodds, PN & Figueroa, M 2019, 'Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation', Nature Communications, vol. 10, no. 1, 5068. https://doi.org/10.1038/s41467-019-12927-7

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T1 - Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

AU - Li, Feng

AU - Upadhyaya, Narayana M.

AU - Sperschneider, Jana

AU - Matny, Oadi

AU - Nguyen-Phuc, Hoa

AU - Mago, Rohit

AU - Raley, Castle

AU - Miller, Marisa E.

AU - Silverstein, Kevin A.T.

AU - Henningsen, Eva

AU - Hirsch, Cory D.

AU - Visser, Botma

AU - Pretorius, Zacharias A.

AU - Steffenson, Brian J.

AU - Schwessinger, Benjamin

AU - Dodds, Peter N.

AU - Figueroa, Melania

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Parasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However, non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), such as Ug99, are a major threat to global food security. Here, we provide genomics-based evidence supporting that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons. Fully haplotype-resolved genome assembly and DNA proximity analysis reveal that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt, with no recombination or chromosome reassortment. These findings indicate that nuclear exchange between dikaryotes can generate genetic diversity and facilitate the emergence of new lineages in asexual fungal populations.

AB - Parasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However, non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), such as Ug99, are a major threat to global food security. Here, we provide genomics-based evidence supporting that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons. Fully haplotype-resolved genome assembly and DNA proximity analysis reveal that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt, with no recombination or chromosome reassortment. These findings indicate that nuclear exchange between dikaryotes can generate genetic diversity and facilitate the emergence of new lineages in asexual fungal populations.

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JO - Nature Communications

JF - Nature Communications

IS - 1

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ER -

Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

Abstract

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