Endoplasmic reticulum targeted GFP reveals ER organization in tobacco NT-1 cells during cell division

S. L. Gupton, D. A. Collings, N. S. Allen*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    50 Citations (Scopus)

    Abstract

    The endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microscopy. During division, the ER and nuclear envelope do not vesiculate. Instead, tubules of ER accumulate around the chromosomes after the nuclear envelope breaks down, with these tubules aligning parallel to the microtubules of the mitotic spindle. In cytokinesis, the phragmoplast is particularly rich in ER, and the transnuclear channels and invaginations present in many interphase cells appear to develop from ER tubules trapped in the developing phragmoplast. Drug studies, using oryzalin and latrunculin to disrupt the microtubules and actin microfilaments, respectively, demonstrate that during division, the arrangement of ER is controlled by microtubules and not by actin, which is the reverse of the situation in interphase cells.

    Original languageEnglish
    Pages (from-to)95-105
    Number of pages11
    JournalPlant Physiology and Biochemistry
    Volume44
    Issue number2-3
    DOIs
    Publication statusPublished - Feb 2006

    Fingerprint

    Dive into the research topics of 'Endoplasmic reticulum targeted GFP reveals ER organization in tobacco NT-1 cells during cell division'. Together they form a unique fingerprint.

    Cite this