TY - JOUR
T1 - Endoplasmic reticulum targeted GFP reveals ER organization in tobacco NT-1 cells during cell division
AU - Gupton, S. L.
AU - Collings, D. A.
AU - Allen, N. S.
PY - 2006/2
Y1 - 2006/2
N2 - The endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microscopy. During division, the ER and nuclear envelope do not vesiculate. Instead, tubules of ER accumulate around the chromosomes after the nuclear envelope breaks down, with these tubules aligning parallel to the microtubules of the mitotic spindle. In cytokinesis, the phragmoplast is particularly rich in ER, and the transnuclear channels and invaginations present in many interphase cells appear to develop from ER tubules trapped in the developing phragmoplast. Drug studies, using oryzalin and latrunculin to disrupt the microtubules and actin microfilaments, respectively, demonstrate that during division, the arrangement of ER is controlled by microtubules and not by actin, which is the reverse of the situation in interphase cells.
AB - The endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microscopy. During division, the ER and nuclear envelope do not vesiculate. Instead, tubules of ER accumulate around the chromosomes after the nuclear envelope breaks down, with these tubules aligning parallel to the microtubules of the mitotic spindle. In cytokinesis, the phragmoplast is particularly rich in ER, and the transnuclear channels and invaginations present in many interphase cells appear to develop from ER tubules trapped in the developing phragmoplast. Drug studies, using oryzalin and latrunculin to disrupt the microtubules and actin microfilaments, respectively, demonstrate that during division, the arrangement of ER is controlled by microtubules and not by actin, which is the reverse of the situation in interphase cells.
KW - Actin microfilaments
KW - Cell division
KW - Endoplasmic reticulum
KW - Green fluorescent protein
KW - Microtubules
KW - Nuclear envelope
KW - Nuclear invaginations
UR - http://www.scopus.com/inward/record.url?scp=33744511533&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2006.03.003
DO - 10.1016/j.plaphy.2006.03.003
M3 - Article
SN - 0981-9428
VL - 44
SP - 95
EP - 105
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
IS - 2-3
ER -