Environmental sampling for avian influenza virus a (H5N1) in live-bird markets, Indonesia

Risa Indriani, Gina Samaan*, Anita Gultom, Leo Loth, Sri Irianti, Rma Adjid, Ni Luh Putu Indi Dharmayanti, John Weaver, Elizabeth Mumford, Kamalini Lokuge, Paul M. Kelly, Darminto

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    112 Citations (Scopus)

    Abstract

    To identify environmental sites commonly contaminated by avian influenza virus A (H5N1) in live-bird markets in Indonesia, we investigated 83 markets in 3 provinces in Indonesia. At each market, samples were collected from up to 27 poultry-related sites to assess the extent of contamination. Samples were tested by using real-time reverse transcription-PCR and virus isolation. A questionnaire was used to ascertain types of birds in the market, general infrastructure, and work practices. Thirty-nine (47%) markets showed contamination with avian influenza virus in >1 of the sites sampled. Risk factors were slaughtering birds in the market and being located in West Java province. Protective factors included daily removal of waste and zoning that segregated poultry-related work flow areas. These results can aid in the design of evidence-based programs concerning environmental sanitation, food safety, and surveillance to reduce the risk for avian influenza virus A (H5N1) transmission in live-bird markets.

    Original languageEnglish
    Pages (from-to)1889-1895
    Number of pages7
    JournalEmerging Infectious Diseases
    Volume16
    Issue number12
    DOIs
    Publication statusPublished - 1 Dec 2010

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