Establishing reliable production of the PET isotope 89Zr for research use: From target fabrication to preclinical imaging

R. K. Scharli*, R. I. Price, S. Chan, D. Cryer, C. M. Jeffery, A. H. Asad, L. Morandeau, P. Eu, C. Cullinane, A. Kasbollah, A. Katsifis

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    7 Citations (Scopus)

    Abstract

    A semi-automated, in-house external beamline, ≤40 μA at 11.7 MeV for 120 min (degraded from 18 MeV to suppress 88Y & 88Zr co-production) produced 89Zr from 89Y(p,n)89Zr. EOB activity (by HPGe γ-spectr.) of 89Zr in target discs, derived from multiple runs, was 1.42 GBq (±0.45 GBq [SD], n=4) which was 67% (±21%, n=4) of the theoretical activity, with a maximum of 1.84 GBq (87% of theory) achieved. Recovery was 88% (±9%, n=4), radionuclidic purity >99% (n=4) and chemical purity 0.2 ppm Zr (±0.3 ppm, n=3, ICP-MS). The Zr:Y ratio improved from 1:10000 in the pre-filtered solution to 1:10 in the product purified by hydroxamate column. Efficiency of radiolabeling to monoclonal antibody (mAb; trastuzumab) was 100% and purified 89Zr did not bind non-specifically to mAb. Chelator:mAb ratio was 1.3:1. No-carrier-added specific activity of purified 89Zr was 408 MBq/μg (±26 MBq/μg, n=2) via the titration-by-chelator method. Minimum ligand concentration for which 100% labeling occurred was 302 nmol/L. Small animal PET imaging (Philips Mosaic; scan acquisition time 10 min; decay & randoms corrected; image reconstructed using a 3-D RAMLA algorithm) demonstrated marked tumor-specific uptake of 89Zr-labeled mAb but nil 'free' 89Zr (as chloride) tumor uptake.

    Original languageEnglish
    Title of host publication14th International Workshop on Targetry and Target Chemistry
    Pages101-107
    Number of pages7
    DOIs
    Publication statusPublished - 2012
    Event14th International Workshop on Targetry and Target Chemistry, WTTC 2012 - Playa del Carmen, Mexico
    Duration: 26 Aug 201229 Aug 2012

    Publication series

    NameAIP Conference Proceedings
    Volume1509
    ISSN (Print)0094-243X
    ISSN (Electronic)1551-7616

    Conference

    Conference14th International Workshop on Targetry and Target Chemistry, WTTC 2012
    Country/TerritoryMexico
    CityPlaya del Carmen
    Period26/08/1229/08/12

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