Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination

Zheyi Li; Sreeja Roy; Charani Ranasinghe

doi:10.1007/978-1-0716-2168-4_8

Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination

Zheyi Li, Sreeja Roy, Charani Ranasinghe^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Recently, we have shown that fate of a vaccine is determined by the cytokine milieu in the innate immune compartment at the early stage of vaccination. Specifically, 24 h post-delivery, level of innate lymphoid cell type 2 (ILC2)-derived IL-13/IL-13Rα2 are the master regulators of DC and also different ILC subsets responsible for modulating the downstream immune outcomes. Here, we provide step-by-step details how to assess different ILC and DC subsets in lung and muscle following intranasal and intramuscular viral vector vaccination, respectively, using multi-color flow cytometry and confocal microscopy.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	137-153
Number of pages	17
DOIs	https://doi.org/10.1007/978-1-0716-2168-4_8
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2465
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/978-1-0716-2168-4_8

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title = "Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination",

abstract = "Recently, we have shown that fate of a vaccine is determined by the cytokine milieu in the innate immune compartment at the early stage of vaccination. Specifically, 24 h post-delivery, level of innate lymphoid cell type 2 (ILC2)-derived IL-13/IL-13Rα2 are the master regulators of DC and also different ILC subsets responsible for modulating the downstream immune outcomes. Here, we provide step-by-step details how to assess different ILC and DC subsets in lung and muscle following intranasal and intramuscular viral vector vaccination, respectively, using multi-color flow cytometry and confocal microscopy.",

keywords = "Confocal microscopy, Dendritic cells (DC), Flow cytometry, IL-4/IL-13 regulation, Innate lymphoid cells (ILC), Intranasal (i.n.)/intramuscular (i.m.) vaccination, Lung and muscle single cell preparation, Recombinant fowl poxvirus (rFPV), Viral vector vaccination",

author = "Zheyi Li and Sreeja Roy and Charani Ranasinghe",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2168-4_8",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

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T1 - Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination

AU - Li, Zheyi

AU - Roy, Sreeja

AU - Ranasinghe, Charani

PY - 2022

Y1 - 2022

N2 - Recently, we have shown that fate of a vaccine is determined by the cytokine milieu in the innate immune compartment at the early stage of vaccination. Specifically, 24 h post-delivery, level of innate lymphoid cell type 2 (ILC2)-derived IL-13/IL-13Rα2 are the master regulators of DC and also different ILC subsets responsible for modulating the downstream immune outcomes. Here, we provide step-by-step details how to assess different ILC and DC subsets in lung and muscle following intranasal and intramuscular viral vector vaccination, respectively, using multi-color flow cytometry and confocal microscopy.

AB - Recently, we have shown that fate of a vaccine is determined by the cytokine milieu in the innate immune compartment at the early stage of vaccination. Specifically, 24 h post-delivery, level of innate lymphoid cell type 2 (ILC2)-derived IL-13/IL-13Rα2 are the master regulators of DC and also different ILC subsets responsible for modulating the downstream immune outcomes. Here, we provide step-by-step details how to assess different ILC and DC subsets in lung and muscle following intranasal and intramuscular viral vector vaccination, respectively, using multi-color flow cytometry and confocal microscopy.

KW - Confocal microscopy

KW - Dendritic cells (DC)

KW - Flow cytometry

KW - IL-4/IL-13 regulation

KW - Innate lymphoid cells (ILC)

KW - Intranasal (i.n.)/intramuscular (i.m.) vaccination

KW - Lung and muscle single cell preparation

KW - Recombinant fowl poxvirus (rFPV)

KW - Viral vector vaccination

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U2 - 10.1007/978-1-0716-2168-4_8

DO - 10.1007/978-1-0716-2168-4_8

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 137

EP - 153

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination

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