Extramedullary hematopoiesis: mesenchymal stromal cells from spleen provide an in vitro niche for myelopoiesis

Sawang Petvises, Vinson Tran, Ying Ying Hey, Dipti Talaulikar, Terence J. O’Neill, Jonathan Tan, Helen C. O’Neill*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Murine spleen has been shown to harbour stromal cells that support hematopoiesis with production of myeloid antigen–presenting cells. Similar stromal lines have now been isolated from long-term cultures (LTC) of human spleen. When human progenitor populations from spleen, bone marrow and cord blood were employed as a source of progenitors for co-culture above splenic stromal lines, myelopoiesis was supported. Human splenocytes gave production of predominantly myeloid dendritic-like cells, with minor subsets resembling conventional dendritic cells (cDC) cells, and myeloid or monocyte-derived DC. Human bone marrow progenitors gave rise to myelopoiesis from hematopoietic progenitors, while human cord blood supported limited myelopoiesis from existing myeloid precursors. Transcriptome analysis compared two stromal lines differing in myelopoietic support capacity. Gene profiling revealed both stromal lines to reflect perivascular reticular cells with osteogenic characteristics. However, the 5C6 stroma which failed to support hematopoiesis uniquely expressed several inhibitors of the WNT pathway. Combined data now show that splenic stroma of both human and murine origin provides a mesenchymal stromal cell microenvironment which is WNT pathway–dependent, and which supports in vitro myelopoiesis with production of specific subsets of myeloid and dendritic-like cells.

    Original languageEnglish
    Pages (from-to)429-439
    Number of pages11
    JournalIn Vitro Cellular and Developmental Biology - Animal
    Volume58
    Issue number5
    DOIs
    Publication statusPublished - May 2022

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