TY - JOUR
T1 - Fatty acid profiling of Chlamydomonas reinhardtii under nitrogen deprivation
AU - James, Gabriel O.
AU - Hocart, Charles H.
AU - Hillier, Warwick
AU - Chen, Hancai
AU - Kordbacheh, Farzaneh
AU - Price, G. Dean
AU - Djordjevic, Michael A.
PY - 2011/2
Y1 - 2011/2
N2 - The Chlamydomonas reinhardtii starch-less mutant, BAF-J5, was found to store lipids up to 65% of dry cell weight when grown photoheterotrophically and subjected to nitrogen starvation. Fourier transform infrared spectroscopy was used as a high-throughput method for semi-quantitative measurements of protein, carbohydrate and lipid content. The fatty acids of wild-type and starch mutants were identified and quantified by gas chromatography mass spectrometry. C. reinhardtii starch mutants, BAF-J5 and I7, produce significantly elevated levels of 16:0, 18:1Δ9, 18:2Δ9,12 and 18:3Δ9,12,15 fatty acids. Long-chain saturated, mono- and polyunsaturated fatty acids were found under nitrogen starvation. Oleosin-like and caleosin-like genes were identified in the C. reinhardtii genome. However, proteomic analysis of isolated lipid bodies only identified a key lipid droplet associated protein. This study shows it is possible to manipulate algal biosynthetic pathways to produce high levels of lipid that may be suitable for conversion to liquid fuels.
AB - The Chlamydomonas reinhardtii starch-less mutant, BAF-J5, was found to store lipids up to 65% of dry cell weight when grown photoheterotrophically and subjected to nitrogen starvation. Fourier transform infrared spectroscopy was used as a high-throughput method for semi-quantitative measurements of protein, carbohydrate and lipid content. The fatty acids of wild-type and starch mutants were identified and quantified by gas chromatography mass spectrometry. C. reinhardtii starch mutants, BAF-J5 and I7, produce significantly elevated levels of 16:0, 18:1Δ9, 18:2Δ9,12 and 18:3Δ9,12,15 fatty acids. Long-chain saturated, mono- and polyunsaturated fatty acids were found under nitrogen starvation. Oleosin-like and caleosin-like genes were identified in the C. reinhardtii genome. However, proteomic analysis of isolated lipid bodies only identified a key lipid droplet associated protein. This study shows it is possible to manipulate algal biosynthetic pathways to produce high levels of lipid that may be suitable for conversion to liquid fuels.
KW - Biofuel
KW - Chlamydomonas reinhardtii
KW - Fatty acid
KW - GC/MS
KW - Neutral lipid
UR - http://www.scopus.com/inward/record.url?scp=78650826459&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2010.11.051
DO - 10.1016/j.biortech.2010.11.051
M3 - Article
SN - 0960-8524
VL - 102
SP - 3343
EP - 3351
JO - Bioresource Technology
JF - Bioresource Technology
IS - 3
ER -