Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals:

Salah Awel; Richard A. Kirian; Max O. Wiedorn; Kenneth R. Beyerlein; Nils Roth; Daniel A. Horke; Dominik Oberthür; Juraj Knoska; Valerio Mariani; Andrew Morgan; Luigi Adriano; Alexandra Tolstikova; P. Lourdu Xavier; Oleksandr Yefanov; Andrew Aquila; Anton Barty; Shatabdi Roy-Chowdhury; Mark S. Hunter; Daniel James; Joseph S. Robinson; Uwe Weierstall; Andrei V. Rode; Saša Bajt; Jochen Küpper; Henry N. Chapman

doi:10.1107/S1600576717018131

Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals:

Salah Awel, Richard A. Kirian, Max O. Wiedorn, Kenneth R. Beyerlein, Nils Roth, Daniel A. Horke, Dominik Oberthür, Juraj Knoska, Valerio Mariani, Andrew Morgan, Luigi Adriano, Alexandra Tolstikova, P. Lourdu Xavier, Oleksandr Yefanov, Andrew Aquila, Anton Barty, Shatabdi Roy-Chowdhury, Mark S. Hunter, Daniel James, Joseph S. RobinsonUwe Weierstall, Andrei V. Rode, Saša Bajt, Jochen Küpper, Henry N. Chapman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

Original language	English
Pages (from-to)	133-139
Number of pages	7
Journal	Journal of Applied Crystallography
Volume	51
Issue number	1
DOIs	https://doi.org/10.1107/S1600576717018131
Publication status	Published - Feb 2018

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Awel, S., Kirian, R. A., Wiedorn, M. O., Beyerlein, K. R., Roth, N., Horke, D. A., Oberthür, D., Knoska, J., Mariani, V., Morgan, A., Adriano, L., Tolstikova, A., Xavier, P. L., Yefanov, O., Aquila, A., Barty, A., Roy-Chowdhury, S., Hunter, M. S., James, D., ... Chapman, H. N. (2018). Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals: Journal of Applied Crystallography, 51(1), 133-139. https://doi.org/10.1107/S1600576717018131

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title = "Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals:",

abstract = "High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.",

keywords = "X-ray diffraction, aerosols, nanocrystals",

author = "Salah Awel and Kirian, {Richard A.} and Wiedorn, {Max O.} and Beyerlein, {Kenneth R.} and Nils Roth and Horke, {Daniel A.} and Dominik Oberth{\"u}r and Juraj Knoska and Valerio Mariani and Andrew Morgan and Luigi Adriano and Alexandra Tolstikova and Xavier, {P. Lourdu} and Oleksandr Yefanov and Andrew Aquila and Anton Barty and Shatabdi Roy-Chowdhury and Hunter, {Mark S.} and Daniel James and Robinson, {Joseph S.} and Uwe Weierstall and Rode, {Andrei V.} and Sa{\v s}a Bajt and Jochen K{\"u}pper and Chapman, {Henry N.}",

note = "Publisher Copyright: {\textcopyright} Salah Awel et al. 2018.",

year = "2018",

month = feb,

doi = "10.1107/S1600576717018131",

language = "English",

volume = "51",

pages = "133--139",

journal = "Journal of Applied Crystallography",

issn = "0021-8898",

publisher = "International Union of Crystallography",

number = "1",

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Awel, S, Kirian, RA, Wiedorn, MO, Beyerlein, KR, Roth, N, Horke, DA, Oberthür, D, Knoska, J, Mariani, V, Morgan, A, Adriano, L, Tolstikova, A, Xavier, PL, Yefanov, O, Aquila, A, Barty, A, Roy-Chowdhury, S, Hunter, MS, James, D, Robinson, JS, Weierstall, U, Rode, AV, Bajt, S, Küpper, J & Chapman, HN 2018, 'Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals:', Journal of Applied Crystallography, vol. 51, no. 1, pp. 133-139. https://doi.org/10.1107/S1600576717018131

TY - JOUR

T1 - Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals:

AU - Awel, Salah

AU - Kirian, Richard A.

AU - Wiedorn, Max O.

AU - Beyerlein, Kenneth R.

AU - Roth, Nils

AU - Horke, Daniel A.

AU - Oberthür, Dominik

AU - Knoska, Juraj

AU - Mariani, Valerio

AU - Morgan, Andrew

AU - Adriano, Luigi

AU - Tolstikova, Alexandra

AU - Xavier, P. Lourdu

AU - Yefanov, Oleksandr

AU - Aquila, Andrew

AU - Barty, Anton

AU - Roy-Chowdhury, Shatabdi

AU - Hunter, Mark S.

AU - James, Daniel

AU - Robinson, Joseph S.

AU - Weierstall, Uwe

AU - Rode, Andrei V.

AU - Bajt, Saša

AU - Küpper, Jochen

AU - Chapman, Henry N.

PY - 2018/2

Y1 - 2018/2

N2 - High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

AB - High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

KW - X-ray diffraction

KW - aerosols

KW - nanocrystals

UR - http://www.scopus.com/inward/record.url?scp=85041426922&partnerID=8YFLogxK

U2 - 10.1107/S1600576717018131

DO - 10.1107/S1600576717018131

M3 - Article

SN - 0021-8898

VL - 51

SP - 133

EP - 139

JO - Journal of Applied Crystallography

JF - Journal of Applied Crystallography

IS - 1

ER -

Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals:

Abstract

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