FITC-functionalized TiO2 nanoparticles for simultaneous neuron imaging and in cell photocatalysis

Tina Zhang; Mary Ann Go; Christian Stricker; Vincent Daria; Antonio Tricoli

doi:10.1557/opl.2014.893

FITC-functionalized TiO₂ nanoparticles for simultaneous neuron imaging and in cell photocatalysis

Tina Zhang, Mary Ann Go, Christian Stricker, Vincent Daria, Antonio Tricoli

Research output: Contribution to journal › Conference article › peer-review

Abstract

Crystalline TiO₂ nanoparticles were produced by scalable flame spray pyrolysis of organometallic solutions. A protocol is presented for the optimized functionalization of these particles with fluorescein isothiocyanate (FITC), an important biomedical dye via a lysine linker. The pH, stoichiometry and time for lysine reaction were determined for highest dye loading and minimized degree of polylysine formation. Acidic reaction conditions, low lysine concentration and short reaction times were found to meet this aim. The resulting particles were used for imaging single neurons, showing high fluorescence emission and ability for the particles to diffuse into small neuron structures such as dendrites.

Original language	English
Article number	893
Journal	Materials Research Society Symposium - Proceedings
Volume	1694
DOIs	https://doi.org/10.1557/opl.2014.893
Publication status	Published - 2014
Event	2014 MRS Spring Meeting - San Francisco, United States Duration: 21 Apr 2014 → 25 Apr 2014

Access to Document

10.1557/opl.2014.893

Cite this

@article{973e050199044149a0afe0ec17bae5fa,

title = "FITC-functionalized TiO2 nanoparticles for simultaneous neuron imaging and in cell photocatalysis",

abstract = "Crystalline TiO2 nanoparticles were produced by scalable flame spray pyrolysis of organometallic solutions. A protocol is presented for the optimized functionalization of these particles with fluorescein isothiocyanate (FITC), an important biomedical dye via a lysine linker. The pH, stoichiometry and time for lysine reaction were determined for highest dye loading and minimized degree of polylysine formation. Acidic reaction conditions, low lysine concentration and short reaction times were found to meet this aim. The resulting particles were used for imaging single neurons, showing high fluorescence emission and ability for the particles to diffuse into small neuron structures such as dendrites.",

keywords = "catalytic, composite, flame synthesis",

author = "Tina Zhang and Go, {Mary Ann} and Christian Stricker and Vincent Daria and Antonio Tricoli",

note = "Publisher Copyright: Copyright {\textcopyright} 2014 Materials Research Society.; 2014 MRS Spring Meeting ; Conference date: 21-04-2014 Through 25-04-2014",

year = "2014",

doi = "10.1557/opl.2014.893",

language = "English",

volume = "1694",

journal = "Materials Research Society Symposium - Proceedings",

issn = "0272-9172",

publisher = "Materials Research Society",

}

TY - JOUR

T1 - FITC-functionalized TiO2 nanoparticles for simultaneous neuron imaging and in cell photocatalysis

AU - Zhang, Tina

AU - Go, Mary Ann

AU - Stricker, Christian

AU - Daria, Vincent

AU - Tricoli, Antonio

PY - 2014

Y1 - 2014

N2 - Crystalline TiO2 nanoparticles were produced by scalable flame spray pyrolysis of organometallic solutions. A protocol is presented for the optimized functionalization of these particles with fluorescein isothiocyanate (FITC), an important biomedical dye via a lysine linker. The pH, stoichiometry and time for lysine reaction were determined for highest dye loading and minimized degree of polylysine formation. Acidic reaction conditions, low lysine concentration and short reaction times were found to meet this aim. The resulting particles were used for imaging single neurons, showing high fluorescence emission and ability for the particles to diffuse into small neuron structures such as dendrites.

AB - Crystalline TiO2 nanoparticles were produced by scalable flame spray pyrolysis of organometallic solutions. A protocol is presented for the optimized functionalization of these particles with fluorescein isothiocyanate (FITC), an important biomedical dye via a lysine linker. The pH, stoichiometry and time for lysine reaction were determined for highest dye loading and minimized degree of polylysine formation. Acidic reaction conditions, low lysine concentration and short reaction times were found to meet this aim. The resulting particles were used for imaging single neurons, showing high fluorescence emission and ability for the particles to diffuse into small neuron structures such as dendrites.

KW - catalytic

KW - composite

KW - flame synthesis

UR - http://www.scopus.com/inward/record.url?scp=84924373025&partnerID=8YFLogxK

U2 - 10.1557/opl.2014.893

DO - 10.1557/opl.2014.893

M3 - Conference article

SN - 0272-9172

VL - 1694

JO - Materials Research Society Symposium - Proceedings

JF - Materials Research Society Symposium - Proceedings

M1 - 893

T2 - 2014 MRS Spring Meeting

Y2 - 21 April 2014 through 25 April 2014

ER -

FITC-functionalized TiO₂ nanoparticles for simultaneous neuron imaging and in cell photocatalysis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this