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fluxible: An R package to process ecosystem gas fluxes from closed-loop chambers in an automated and reproducible way

  • Joseph Gaudard*
  • , Richard J. Telford
  • , Julia Chacon-Labella
  • , Hilary Rose Dawson
  • , Brian J. Enquist
  • , Joachim P. Töpper
  • , Jonas Trepel
  • , Vigdis Vandvik
  • , Marta Baumane
  • , Kristine Birkeli
  • , Mukhlish Jamal Musa Holle
  • , Jason R. Hupp
  • , Paul Efren Santos-Andrade
  • , Tin Widyani Satriawan
  • , Aud H. Halbritter
  • *Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

1. Measuring ecosystem gas fluxes is crucial to understanding the dynamics of ecosystem water and energy cycling. A common method to measure ecosystem gas fluxes (CO2, CH4, N2O) and to compare experimental treatments is the use of closed-loop chambers. 

2. However, the output data require extensive processing before analysis, making the workflow prone to bias and limiting comparability between studies. While there are various methods to process flux data, they lack reproducibility and automation. 

3. The fluxible R package provides a reproducible way to process raw gas concentration data from closed-loop chambers into an analysis-ready dataset of ecosystem gas fluxes. The processing steps include (1) separating the measurements, (2) fitting a linear, quadratic, or exponential model, (3) assessing the quality of the fit, (4) plotting the fluxes for visual inspection and (5) calculating the fluxes. 

4. In addition to reproducibility, fluxible focuses on homogeneity and automation in data processing, thus reducing the time investment for users while improving comparability across studies.

Original languageEnglish
Number of pages9
JournalMethods in Ecology and Evolution
Volume16
Issue number11
DOIs
Publication statusE-pub ahead of print - 25 Sept 2025

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