@inproceedings{b05393bbc24a4520933c1026a83b3bfa,
title = "Four-dimensional multi-site two-photon excitation",
abstract = "We use the holographic method to project an arbitrary array of diffraction-limited focal spots suitable for multi-site twophoton excitation. The spot array can be projected arbitrarily within a three-dimensional (3D) volume, while the fourth dimension in time is attributed to high temporal resolution via high-speed non-iterative calculation of the hologram using a video graphics accelerator board. We show that the spots have sufficient energy and spatiotemporal photon density for localized two-photon excitation at individual spots in the array. The significance of this work points to 3D microscopy, non-linear micro-fabrication, volume holographic optical storage and biomedical instrumentation. In neuroscience, timecritical release of neurotransmitters at multiple sites within complex dendritic trees of neurons can lead to insights on the mechanisms of information processing in the brain.",
keywords = "Computer holography, Fluorescence microscopy, Multi-photon processes, Spatial Light Modulator",
author = "Daria, {Vincent R.} and Christian Stricker and Richard Bowman and Bachor, {Hans A.} and Stephen Redman",
year = "2010",
doi = "10.1117/12.840782",
language = "English",
isbn = "9780819479440",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Photonic Therapeutics and Diagnostics VI",
note = "Photonic Therapeutics and Diagnostics VI ; Conference date: 23-01-2010 Through 25-01-2010",
}