Functional CpG methylation system in a social insect

Ying Wang; Mireia Jorda; Peter L. Jones; Rjrszard Maleszka; Xu Ling; Hugh M. Robertson; Craig A. Mizzen; Miguel A. Peinado; Gene E. Robinson

doi:10.1126/science.1135213

Functional CpG methylation system in a social insect

Ying Wang, Mireia Jorda, Peter L. Jones, Rjrszard Maleszka, Xu Ling, Hugh M. Robertson, Craig A. Mizzen, Miguel A. Peinado, Gene E. Robinson^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

274 Citations (Scopus)

Abstract

DNA methylation systems are well characterized in vertebrates, but methylation in Drosophila melanogoster and other invertebrates remains controversial. Using the recently sequenced honey bee genome, we present a bioinformatic, molecular, and biochemical characterization of a functional DNA methylation system in an insect. We report on catalytically active orthologs of the vertebrate DNA methyltransferases Dnmt1 and Dnmt3a and b, two isoforms that contain a methyl-DNA binding domain, genomic 5-methyl-deoxycytosine, and CpG-methylated genes. The honey bee provides an opportunity to study the roles of methylation in social contexts.

Original language	English
Pages (from-to)	645-647
Number of pages	3
Journal	Science
Volume	314
Issue number	5799
DOIs	https://doi.org/10.1126/science.1135213
Publication status	Published - 27 Oct 2006

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title = "Functional CpG methylation system in a social insect",

abstract = "DNA methylation systems are well characterized in vertebrates, but methylation in Drosophila melanogoster and other invertebrates remains controversial. Using the recently sequenced honey bee genome, we present a bioinformatic, molecular, and biochemical characterization of a functional DNA methylation system in an insect. We report on catalytically active orthologs of the vertebrate DNA methyltransferases Dnmt1 and Dnmt3a and b, two isoforms that contain a methyl-DNA binding domain, genomic 5-methyl-deoxycytosine, and CpG-methylated genes. The honey bee provides an opportunity to study the roles of methylation in social contexts.",

author = "Ying Wang and Mireia Jorda and Jones, \{Peter L.\} and Rjrszard Maleszka and Xu Ling and Robertson, \{Hugh M.\} and Mizzen, \{Craig A.\} and Peinado, \{Miguel A.\} and Robinson, \{Gene E.\}",

year = "2006",

month = oct,

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doi = "10.1126/science.1135213",

language = "English",

volume = "314",

pages = "645--647",

journal = "Science",

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T1 - Functional CpG methylation system in a social insect

AU - Wang, Ying

AU - Jorda, Mireia

AU - Jones, Peter L.

AU - Maleszka, Rjrszard

AU - Ling, Xu

AU - Robertson, Hugh M.

AU - Mizzen, Craig A.

AU - Peinado, Miguel A.

AU - Robinson, Gene E.

PY - 2006/10/27

Y1 - 2006/10/27

N2 - DNA methylation systems are well characterized in vertebrates, but methylation in Drosophila melanogoster and other invertebrates remains controversial. Using the recently sequenced honey bee genome, we present a bioinformatic, molecular, and biochemical characterization of a functional DNA methylation system in an insect. We report on catalytically active orthologs of the vertebrate DNA methyltransferases Dnmt1 and Dnmt3a and b, two isoforms that contain a methyl-DNA binding domain, genomic 5-methyl-deoxycytosine, and CpG-methylated genes. The honey bee provides an opportunity to study the roles of methylation in social contexts.

AB - DNA methylation systems are well characterized in vertebrates, but methylation in Drosophila melanogoster and other invertebrates remains controversial. Using the recently sequenced honey bee genome, we present a bioinformatic, molecular, and biochemical characterization of a functional DNA methylation system in an insect. We report on catalytically active orthologs of the vertebrate DNA methyltransferases Dnmt1 and Dnmt3a and b, two isoforms that contain a methyl-DNA binding domain, genomic 5-methyl-deoxycytosine, and CpG-methylated genes. The honey bee provides an opportunity to study the roles of methylation in social contexts.

UR - https://www.scopus.com/pages/publications/33750486851

U2 - 10.1126/science.1135213

DO - 10.1126/science.1135213

M3 - Article

SN - 0036-8075

VL - 314

SP - 645

EP - 647

JO - Science

JF - Science

IS - 5799

ER -

Functional CpG methylation system in a social insect

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