Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices

Erez Matalon; Thomas Huber; Gregor Hagelueken; Bim Graham; Veronica Frydman; Akiva Feintuch; Gottfried Otting; Daniella Goldfarb

doi:10.1002/anie.201305574

Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices

Erez Matalon, Thomas Huber, Gregor Hagelueken, Bim Graham, Veronica Frydman, Akiva Feintuch, Gottfried Otting, Daniella Goldfarb

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

High-frequency double electron-electron resonance (DEER) distance measurements using different Gd³⁺ tags (Gd-DOTA and Gd-C1) were carried out on transmembrane helical peptides (ca. 0.15-nmol; WALP peptides) in a model membrane. The ability to pick up small distance variations, the chemical flexibility of the tags, and the remarkable absolute sensitivity, make this approach attractive for studies of membrane proteins.

Original language	English
Pages (from-to)	11831-11834
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	45
DOIs	https://doi.org/10.1002/anie.201305574
Publication status	Published - 4 Nov 2013

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title = "Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices",

abstract = "High-frequency double electron-electron resonance (DEER) distance measurements using different Gd3+ tags (Gd-DOTA and Gd-C1) were carried out on transmembrane helical peptides (ca. 0.15-nmol; WALP peptides) in a model membrane. The ability to pick up small distance variations, the chemical flexibility of the tags, and the remarkable absolute sensitivity, make this approach attractive for studies of membrane proteins.",

keywords = "DEER, EPR spectroscopy, gadolinium, membrane proteins, spin labeling",

author = "Erez Matalon and Thomas Huber and Gregor Hagelueken and Bim Graham and Veronica Frydman and Akiva Feintuch and Gottfried Otting and Daniella Goldfarb",

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T1 - Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices

AU - Matalon, Erez

AU - Huber, Thomas

AU - Hagelueken, Gregor

AU - Graham, Bim

AU - Frydman, Veronica

AU - Feintuch, Akiva

AU - Otting, Gottfried

AU - Goldfarb, Daniella

PY - 2013/11/4

Y1 - 2013/11/4

N2 - High-frequency double electron-electron resonance (DEER) distance measurements using different Gd3+ tags (Gd-DOTA and Gd-C1) were carried out on transmembrane helical peptides (ca. 0.15-nmol; WALP peptides) in a model membrane. The ability to pick up small distance variations, the chemical flexibility of the tags, and the remarkable absolute sensitivity, make this approach attractive for studies of membrane proteins.

AB - High-frequency double electron-electron resonance (DEER) distance measurements using different Gd3+ tags (Gd-DOTA and Gd-C1) were carried out on transmembrane helical peptides (ca. 0.15-nmol; WALP peptides) in a model membrane. The ability to pick up small distance variations, the chemical flexibility of the tags, and the remarkable absolute sensitivity, make this approach attractive for studies of membrane proteins.

KW - DEER

KW - EPR spectroscopy

KW - gadolinium

KW - membrane proteins

KW - spin labeling

UR - http://www.scopus.com/inward/record.url?scp=84886781532&partnerID=8YFLogxK

U2 - 10.1002/anie.201305574

DO - 10.1002/anie.201305574

M3 - Article

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 45

ER -

Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices

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