Generation of loss-of-function mutants for wheat rust disease resistance gene cloning

Rohit Mago*, Bradley Till, Sambasivam Periyannan, Guotai Yu, Brande B.H. Wulff, Evans Lagudah

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    15 Citations (Scopus)

    Abstract

    One of the most important tools to identify and validate rust resistance gene function is by producing lossof- function mutants. Mutants can be produced using irradiation, chemicals, and insertions. Among all the mutagens, ethyl methanesulfonate (EMS) and sodium azide are most favored because of the ease of use and generation of random point mutations in the genome. The mutants so produced facilitate the isolation, identification and cloning of rust resistance genes. In this chapter we describe a protocol for seed mutagenesis of wheat with EMS and sodium azide.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages199-205
    Number of pages7
    DOIs
    Publication statusPublished - 2017

    Publication series

    NameMethods in Molecular Biology
    Volume1659
    ISSN (Print)1064-3745

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